E-Cadherin antibody [4A2]

Cat. No. GTX34679

Host	Mouse
Clonality	Monoclonal
Clone Name	4A2
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, FACS
Reactivity	Human
Package	100 μg ($529)

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Datasheet
Document

View product citations for antibody GTX34679 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1-2μg/ml
ICC/IF	1-4μg/ml
IHC-P	1-2μg/ml for 30 minutes at RT
FACS	1-2μg/10⁶ cells
Note : IHC-P Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

97 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human E-Cadherin protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Arc1 , CD324 , CDH1 , CDHE , ECAD , LCAM , UVO , cadherin 1 , E-Cadherin , Cadherin 1

Cellular Localization

Cell junction,Cell membrane,Endosome,Golgi apparatus,trans-Golgi network

Background

This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16. [provided by RefSeq, Nov 2015]

Database

Gene ID: 999 CDH1
UniProt: P12830 CDH1

Research Area

DATA IMAGES

GTX34679 WB Image

WB analysis of MCF-7 cell lysate using GTX34679 E-Cadherin antibody [4A2].

GTX34679 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX34679 E-Cadherin antibody [4A2].
Green : Primary antibody
Red : nucleus

GTX34679 IHC-P Image

IHC-P analysis of human skin tissue using GTX34679 E-Cadherin antibody [4A2].

GTX34679 FACS Image

FACS analysis of trypsinized MCF-7 cells using GTX34679 E-Cadherin antibody [4A2].
Blue : Primary antibody
Red : Isotype control

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 529

Qty