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E-Cadherin antibody [4A2]

Anti-E-Cadherin antibody [4A2] used in IHC (Paraffin sections) (IHC-P). GTX34679
Anti-E-Cadherin antibody [4A2] used in Western Blot (WB). GTX34679
Anti-E-Cadherin antibody [4A2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34679
Anti-E-Cadherin antibody [4A2] used in Flow cytometry (FACS). GTX34679

Cat No. GTX34679

Host

Mouse

Clonality

Monoclonal

Clone Name

4A2

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, FACS

Reactivity

Human
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-2μg/ml
ICC/IF 1-4μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

97 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human E-Cadherin protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Arc1 , CD324 , CDH1 , CDHE , ECAD , LCAM , UVO , cadherin 1 , E-Cadherin , Cadherin 1

Cellular Localization

Cell junction,Cell membrane,Endosome,Golgi apparatus,trans-Golgi network

Background

This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16. [provided by RefSeq, Nov 2015]

Database

Research Area

DATA IMAGES

Anti-E-Cadherin antibody [4A2] used in IHC (Paraffin sections) (IHC-P). GTX34679

GTX34679 IHC-P Image

IHC-P analysis of human skin tissue using GTX34679 E-Cadherin antibody [4A2].

Anti-E-Cadherin antibody [4A2] used in Western Blot (WB). GTX34679

GTX34679 WB Image

WB analysis of MCF-7 cell lysate using GTX34679 E-Cadherin antibody [4A2].

Anti-E-Cadherin antibody [4A2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34679

GTX34679 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX34679 E-Cadherin antibody [4A2].
Green : Primary antibody
Red : nucleus

Anti-E-Cadherin antibody [4A2] used in Flow cytometry (FACS). GTX34679

GTX34679 FACS Image

FACS analysis of trypsinized MCF-7 cells using GTX34679 E-Cadherin antibody [4A2].
Blue : Primary antibody
Red : Isotype control

REFERENCE

There are currently no references for E-Cadherin antibody [4A2] (GTX34679). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for E-Cadherin antibody [4A2] (GTX34679). Be the first to share your experience with this product.
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339