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E-Cadherin antibody [7H12]

Anti-E-Cadherin antibody [7H12] used in Flow cytometry (FACS). GTX82753
Anti-E-Cadherin antibody [7H12] used in Western Blot (WB). GTX82753
Anti-E-Cadherin antibody [7H12] used in IHC (Paraffin sections) (IHC-P). GTX82753

Cat No. GTX82753

Host

Mouse

Clonality

Monoclonal

Clone Name

7H12

Isotype

IgG1

Application

WB, IHC-P, FACS, ELISA

Reactivity

Human, Mouse, Rat, Monkey
Package
100 μl ($349)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1/500 - 1/2000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

97 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites, 0.03% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human CDH1 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cadherin 1 , Arc-1 , BCDS1 , CD324 , CDHE , ECAD , LCAM , UVO

Cellular Localization

Cell junction,Cell membrane,Endosome

Background

This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-E-Cadherin antibody [7H12] used in Flow cytometry (FACS). GTX82753

GTX82753 FACS Image

FACS analysis of HeLa cells using GTX82753 E-Cadherin antibody [7H12].
Green : E-Cadherin
Purple : negative control

Anti-E-Cadherin antibody [7H12] used in Western Blot (WB). GTX82753

GTX82753 WB Image

WB analysis of LNCAP (1),A431 (2), DU145 (3), PC-3 (4), MCF-7 (5), PC-12 (6), NIH3T3 (7), C6 (8) and COS7 (9) cell lysate using GTX82753 E-Cadherin antibody [7H12].

Anti-E-Cadherin antibody [7H12] used in IHC (Paraffin sections) (IHC-P). GTX82753

GTX82753 IHC-P Image

IHC-P analysis of gastric cancer tissue (left) and lung cancer tissue (right) using GTX82753 E-Cadherin antibody [7H12].

REFERENCE

There are currently no references for E-Cadherin antibody [7H12] (GTX82753). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for E-Cadherin antibody [7H12] (GTX82753). Be the first to share your experience with this product.
SDS
Sodium Azide.pdf