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E-Cadherin antibody [CDH1/1525]

Anti-E-Cadherin antibody [CDH1/1525] used in IHC (Paraffin sections) (IHC-P). GTX34680
Anti-E-Cadherin antibody [CDH1/1525] used in IHC (Paraffin sections) (IHC-P). GTX34680
Anti-E-Cadherin antibody [CDH1/1525] used in IHC (Paraffin sections) (IHC-P). GTX34680
Anti-E-Cadherin antibody [CDH1/1525] used in Western Blot (WB). GTX34680

Cat No. GTX34680

Host Mouse
Clonality Monoclonal
Clone Name CDH1/1525
Isotype IgG1
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1ug/ml
ICC/IF 0.5-1ug/ml
IHC-P 1-2ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

97 kDa. ( Note )

Specificity/Sensitivity

Recognizes a protein of 120-80kDa, identified as E-cadherin. Cadherins comprise a family of Ca2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. The classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as β-catenin, to regulate cadherin function. E-cadherin plays an important role in epithelial cell adhesion. A decreased expression of E-cadherin is associated with metastatic potential and poor prognosis in breast cancer, prostate and esophageal cancer. In combination with p120 Catenin, it is useful for the differentiation between ductal (E-cadherin +) and lobular (E-cadherin -) breast carcinomas. It may also help in diagnosis of mesothelioma.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant human full-length E-Cadherin protein

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cadherin 1,Arc-1,Bcds1,Cd324,Cdhe,Ecad,Lcam,Uvo,Cdh1

Background

This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-E-Cadherin antibody [CDH1/1525] used in IHC (Paraffin sections) (IHC-P). GTX34680

GTX34680 IHC-P Image

Formalin-paraffin human Colon Carcinoma stained with E-Cadherin MAb (CDH1/1525).

Anti-E-Cadherin antibody [CDH1/1525] used in IHC (Paraffin sections) (IHC-P). GTX34680

GTX34680 IHC-P Image

Formalin-fixed, paraffin-embedded human Skin stained with E-Cadherin Monoclonal Antibody (CDH1/1525).

Anti-E-Cadherin antibody [CDH1/1525] used in IHC (Paraffin sections) (IHC-P). GTX34680

GTX34680 IHC-P Image

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with E-Cadherin Monoclonal Antibody (CDH1/1525).

Anti-E-Cadherin antibody [CDH1/1525] used in Western Blot (WB). GTX34680

GTX34680 WB Image

Western Blot Analysis (A) Recombinant Protein (B) human Stomach lysate Using E-Cadherin Monoclonal Antibody (CDH1/1525).

Package List Price ($)
$ 329