E-Cadherin antibody [DECMA-1]

Cat No. GTX11512

Reference 6

Host	Rat
Clonality	Monoclonal
Clone Name	DECMA-1
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, IHC-Fr, IP
Reactivity	Human, Mouse, Bovine, Dog
Package	100 μl ($379)

See all E-Cadherin products

Datasheet
Reference

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	Assay dependent
ICC/IF	1:1,600
IHC-P	Assay dependent
IHC-Fr	Assay dependent
IP	Assay dependent

Not tested in other applications.

Calculated MW

98 kDa. ( Note )

Positive Control

MDCK

Specificity/Sensitivity

Monoclonal Anti-Uvomorulin/E-Cadherin was selected against the mouse cell adhesion molecule uvomorulin/E-Cadherin. The antibody localizes the cell surface glycoprotein uvomorulin/E-cadherin that has been found to be identical to L-CAM, Cell CAM 80/120, and ARC-1. It blocks both the aggregation of mouse embryonal carcinoma cells and the compaction of pre-implantation embryos. The antibody disrupts confluent monolayers of Madin-Darby canine kidney (MDCK) epithelial cells. In indirect immunofluorescent staining of MDCK cells grown in culture, the antibody shows strong staining on the membrane of adjacent cells, after treatment with 0.5% Triton-X 100.

PROPERTIES

Form

Liquid

Buffer

Ascites, 15 mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Mouse

Immunogen

mouse embryonal carcinoma cell line PCC4 Aza R1.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cadherin 1 , AA960649 , ARC-1 , E-cad , Ecad , L-CAM , UVO , Um

Cellular Localization

Cell junction,Cell membrane; Single-pass type I membrane protein,Endosome

Background

Cell-cell interactions during embryonic development and in tissue organization involve specific cell-adhesion molecules (CAMs) that are functionally defined by antibodies that interfere with cell-cell adhesion. CAMs are expressed on early embryonic cells and persist in derivatives of all three germ layers. The best characterized CAMs are N-CAM (neural CAM) and L-CAM (liver CAM). The protein Uvomorulin, initially identified in embryonal carcinoma, is identical to L-CAM, E-Cadherin, Cell CAM 80/120 and ARC-1, each of which have been characterized in different systems. E-Cadherin has been characterized as a 120 kDa cell surface glycoprotein from which an 84 kDa fragment can be released by trypsin digestion in the presence of Ca²⁺.

Database

Gene ID: 12550 Cdh1
UniProt: P09803 Cdh1

Research Area

DATA IMAGES

GTX11512 ICC/IF Image

ICC/IF analysis of MDCK cells using GTX11512 E-Cadherin antibody [DECMA-1] at 1:3200. Cells were fixed and permeabilized with methanol followed by acetone.

GTX11512 ICC/IF Image

ICC/IF analysis of MDCK cells using GTX11512 E-Cadherin antibody [DECMA-1] at 1:3,200. Cells were fixed and permeabilized with cold methanol followed by acetone.