APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
Assay dependent |
1:1,600 |
Assay dependent |
Assay dependent |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
MDCK
Product Note
Monoclonal Anti-Uvomorulin/E-Cadherin was selected against the mouse cell adhesion molecule uvomorulin/E-Cadherin. The antibody localizes the cell surface glycoprotein uvomorulin/E-cadherin that has been found to be identical to L-CAM, Cell CAM 80/120, and ARC-1. It blocks both the aggregation of mouse embryonal carcinoma cells and the compaction of pre-implantation embryos. The antibody disrupts confluent monolayers of Madin-Darby canine kidney (MDCK) epithelial cells. In indirect immunofluorescent staining of MDCK cells grown in culture, the antibody shows strong staining on the membrane of adjacent cells, after treatment with 0.5% Triton-X 100.
PROPERTIES
Form
Liquid
Buffer
Ascites
Preservative
15mM Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Mouse
Immunogen
mouse embryonal carcinoma cell line PCC4 Aza R1.
Purification
Unpurified
Conjugation
Unconjugated
RRID
AB_381324
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
cadherin 1 , AA960649 , ARC-1 , E-cad , Ecad , L-CAM , UVO , Um
Cellular Localization
Cell junction,Cell membrane; Single-pass type I membrane protein,Endosome
Background
Cell-cell interactions during embryonic development and in tissue organization involve specific cell-adhesion molecules (CAMs) that are functionally defined by antibodies that interfere with cell-cell adhesion. CAMs are expressed on early embryonic cells and persist in derivatives of all three germ layers. The best characterized CAMs are N-CAM (neural CAM) and L-CAM (liver CAM). The protein Uvomorulin, initially identified in embryonal carcinoma, is identical to L-CAM, E-Cadherin, Cell CAM 80/120 and ARC-1, each of which have been characterized in different systems. E-Cadherin has been characterized as a 120 kDa cell surface glycoprotein from which an 84 kDa fragment can be released by trypsin digestion in the presence of Ca2+.
Database
Research Area
DATA IMAGES
|
GTX11512 ICC/IF Image
ICC/IF analysis of MDCK cells using GTX11512 E-Cadherin antibody [DECMA-1] at 1:3200. Cells were fixed and permeabilized with methanol followed by acetone.
|
|
GTX11512 ICC/IF Image
ICC/IF analysis of MDCK cells using GTX11512 E-Cadherin antibody [DECMA-1] at 1:3,200. Cells were fixed and permeabilized with cold methanol followed by acetone.
|
|
GTX11512 WB Image
WB analysis of MDCK cells using GTX11512 E-Cadherin antibody [DECMA-1] at 1:250.
|
|
GTX11512 WB Image
The data was published in the journal Int J Mol Sci in 2020.PMID: 31991851
|
|
GTX11512 IHC-Fr Image
The data was published in the journal Int J Mol Sci in 2020.PMID: 31991851
|
|
GTX11512 WB Image
The data was published in the journal Sci Rep in 2019. PMID: 31101909
|
|
GTX11512 ICC/IF Image
The data was published in the journal Nat Commun in 2020.PMID: 31974352
|
|
GTX11512 IHC-P Image
The data was published in the journal Sci Rep in 2019. PMID: 31101909
|
REFERENCE
REVIEW
There are currently no reviews for E-Cadherin antibody [DECMA-1] (GTX11512). Be the first to share your experience with this product.