Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody [GT311] (GTX629692) diluted at 1:2000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.

E-cadherin antibody [GT311] detects E-cadherin protein by Western blot analysis.
A. 30 μg MCF-7 whole cell lysate/extract
B. 30 μg MDA-MB-231 whole cell lysate/extract
5 % SDS-PAGE
E-cadherin antibody [GT311] (GTX629692) dilution: 1:1000

E-adherin antibody [GT311] immunoprecipitates E-adherin protein in IP experiments.
IP samples: MCF-7 whole cell extract
A. 30 μg MCF-7 cell whole cell extract
B. Control with 3 μg of preimmune Mouse IgG
C. Immunoprecipitation of E-adherin protein by 3 μg E-adherin antibody [GT311] (GTX629692)
5 % SDS-PAGE
The immunoprecipitated E-adherin protein was detected by E-adherin antibody [GT311] (GTX629692) diluted at 1:500.
[EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent]

E-Cadherin antibody [GT311] (GTX629692) detects E-Cadherin protein by flow cytometry analysis.
Sample: MCF-7 cell.
Black: Unlabelled sample was used as a control.
Red: E-Cadherin antibody [GT311] (GTX629692) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.