GeneTex
  • Country / Location Selection

United States (US)

E-Cadherin antibody [GT311]

Anti-E-Cadherin antibody [GT311] used in Western Blot (WB). GTX629692
Anti-E-Cadherin antibody [GT311] used in Immunoprecipitation (IP). GTX629692
Anti-E-Cadherin antibody [GT311] used in Western Blot (WB). GTX629692
Anti-E-Cadherin antibody [GT311] used in Flow cytometry (FACS). GTX629692

Cat No. GTX629692

Host

Mouse

Clonality

Monoclonal

Clone Name

GT311

Isotype

IgG1

Application

WB, FACS, IP

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
FACS 1:50-1:200
IP 1:100-1:500
Not tested in other applications.

Calculated MW

97 kDa. ( Note )

Positive Control

MCF-7

Predict Reactivity

Mouse, Rat, Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human E-Cadherin. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888130

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cadherin 1 , Arc-1 , BCDS1 , CD324 , CDHE , ECAD , LCAM , UVO

Cellular Localization

Cell junction , Cell membrane

Background

This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-E-Cadherin antibody [GT311] used in Western Blot (WB). GTX629692

GTX629692 WB Image

Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody [GT311] (GTX629692) diluted at 1:2000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-E-Cadherin antibody [GT311] used in Immunoprecipitation (IP). GTX629692

GTX629692 IP Image

E-adherin antibody [GT311] immunoprecipitates E-adherin protein in IP experiments.
IP samples: MCF-7 whole cell extract
A. 30 μg MCF-7 cell whole cell extract
B. Control with 3 μg of preimmune Mouse IgG
C. Immunoprecipitation of E-adherin protein by 3 μg E-adherin antibody [GT311] (GTX629692)
5 % SDS-PAGE
The immunoprecipitated E-adherin protein was detected by E-adherin antibody [GT311] (GTX629692) diluted at 1:500.
[EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent]

Anti-E-Cadherin antibody [GT311] used in Western Blot (WB). GTX629692

GTX629692 WB Image

E-cadherin antibody [GT311] detects E-cadherin protein by Western blot analysis.
A. 30 μg MCF-7 whole cell lysate/extract
B. 30 μg MDA-MB-231 whole cell lysate/extract
5 % SDS-PAGE
E-cadherin antibody [GT311] (GTX629692) dilution: 1:1000

Anti-E-Cadherin antibody [GT311] used in Flow cytometry (FACS). GTX629692

GTX629692 FACS Image

E-Cadherin antibody [GT311] (GTX629692) detects E-Cadherin protein by flow cytometry analysis.
Sample: MCF-7 cell.
Black: Unlabelled sample was used as a control.
Red: E-Cadherin antibody [GT311] (GTX629692) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.

REFERENCE

There are currently no references for E-Cadherin antibody [GT311] (GTX629692). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for E-Cadherin antibody [GT311] (GTX629692). Be the first to share your experience with this product.
SDS
PBS.pdf