*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
Mouse, Rat, Bovine, Dog, Pig(>80% identity)
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the center region of human E-Cadherin. The exact sequence is proprietary.
Affinity purified by Protein G.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
cadherin 1 , Arc-1 , BCDS1 , CD324 , CDHE , ECAD , LCAM , UVO
Cell junction, Cell membrane,
This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. [provided by RefSeq]
GTX629694 WB Image
Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody [GT358] (GTX629694) diluted at 1:500. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.
GTX629694 ICC/IF Image
E-Cadherin antibody [GT358] detects E-Cadherin protein at cell membrane by immunofluorescent analysis.
Sample: MCF7 cells were fixed in ice-cold MeOH for 5 min.
Green: E-Cadherin protein stained by E-Cadherin antibody [GT358] (GTX629694) diluted at 1:500.
Blue: Hoechst 33342 staining.
GTX629694 WB Image
E-cadherin antibody [GT358] detects E-cadherin protein by Western blot analysis.
A. 30 μg MCF-7 whole cell lysate/extract
B. 30 μg MDA-MB-231 whole cell lysate/extract
7.5 % SDS-PAGE
E-cadherin antibody [GT358] (GTX629694) dilution: 1:1000
GTX629694 FACS Image
E-Cadherin antibody [GT358] (GTX629694) detects E-Cadherin protein by flow cytometry analysis.
Sample: MCF-7 cell.
Black: Unlabelled sample was used as a control.
Red: E-Cadherin antibody [GT358] (GTX629694) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.