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E-Cadherin antibody [GT358]

KO/KD
Anti-E-Cadherin antibody [GT358] used in Western Blot (WB). GTX629694
Anti-E-Cadherin antibody [GT358] used in Western Blot (WB). GTX629694
Anti-E-Cadherin antibody [GT358] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629694
Anti-E-Cadherin antibody [GT358] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629694
Anti-E-Cadherin antibody [GT358] used in Flow cytometry (FACS). GTX629694
Anti-E-Cadherin antibody [GT358] used in Western Blot (WB). GTX629694

Cat. No. GTX629694

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT358

Isotype

 IgG2b

Application

 WB, ICC/IF, FACS

Reactivity

 Human, Dog
Package
100 μl ($399),
25 μl ($169)
See all E-Cadherin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
FACS 1:50-1:200
Not tested in other applications.

Calculated MW

97 kDa. ( Note )

Positive Control

MCF-7 , MDA-MB-231

Predict Reactivity

Mouse, Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human E-Cadherin. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888131

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cadherin 1 , Arc-1 , BCDS1 , CD324 , CDHE , ECAD , LCAM , UVO

Cellular Localization

Cell junction, Cell membrane,

Background

This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-E-Cadherin antibody [GT358] used in Western Blot (WB). GTX629694

GTX629694 WB Image

KO/KD

Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody [GT358] (GTX629694) diluted at 1:500. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-E-Cadherin antibody [GT358] used in Western Blot (WB). GTX629694

GTX629694 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody [GT358] (GTX629694) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-E-Cadherin antibody [GT358] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629694

GTX629694 ICC/IF Image

E-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: E-Cadherin stained by E-Cadherin antibody (GTX629694) diluted at 1:500.

Anti-E-Cadherin antibody [GT358] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629694

GTX629694 ICC/IF Image

E-Cadherin antibody [GT358] detects E-Cadherin protein at cell membrane by immunofluorescent analysis.
Sample: MCF7 cells were fixed in ice-cold MeOH for 5 min.
Green: E-Cadherin protein stained by E-Cadherin antibody [GT358] (GTX629694) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-E-Cadherin antibody [GT358] used in Flow cytometry (FACS). GTX629694

GTX629694 FACS Image

E-Cadherin antibody [GT358] (GTX629694) detects E-Cadherin protein by flow cytometry analysis.
Sample: MCF-7 cell.
Black: Unlabelled sample was used as a control.
Red: E-Cadherin antibody [GT358] (GTX629694) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.

Anti-E-Cadherin antibody [GT358] used in Western Blot (WB). GTX629694

GTX629694 WB Image

E-cadherin antibody [GT358] detects E-cadherin protein by Western blot analysis.
A. 30 μg MCF-7 whole cell lysate/extract
B. 30 μg MDA-MB-231 whole cell lysate/extract
7.5 % SDS-PAGE
E-cadherin antibody [GT358] (GTX629694) dilution: 1:1000

REFERENCE

Application Reference

Lin-Sheng Yu et al. Cancers (Basel) 2021; 13 (16) Tissue Architecture Influences the Biological Effectiveness of Boron Neutron Capture Therapy in In Vitro/In Silico Three-Dimensional Self-Assembly Cell Models of Pancreatic Cancers.
Application : WB, ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations