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E-Cadherin antibody [GT477]

Anti-E-Cadherin antibody [GT477] used in IHC (Paraffin sections) (IHC-P). GTX629691
Anti-E-Cadherin antibody [GT477] used in IHC (Paraffin sections) (IHC-P). GTX629691
Anti-E-Cadherin antibody [GT477] used in Western Blot (WB). GTX629691
Anti-E-Cadherin antibody [GT477] used in Immunoprecipitation (IP). GTX629691
Anti-E-Cadherin antibody [GT477] used in Western Blot (WB). GTX629691
Anti-E-Cadherin antibody [GT477] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629691
Anti-E-Cadherin antibody [GT477] used in Flow cytometry (FACS). GTX629691
Anti-E-Cadherin antibody [GT477] used in Western Blot (WB). GTX629691
Anti-E-Cadherin antibody [GT477] used in Western Blot (WB). GTX629691
Anti-E-Cadherin antibody [GT477] used in Western Blot (WB). GTX629691

Cat No. GTX629691

Host

Mouse

Clonality

Monoclonal

Clone Name

GT477

Isotype

IgG2a

Application

WB, ICC/IF, IHC-P, FACS, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
FACS 1:50-1:200
IP 1:100-1:500
Not tested in other applications.

Calculated MW

97 kDa. ( Note )

Positive Control

MCF-7 , MDA-MB-231

Predict Reactivity

Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human E-Cadherin. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888129

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cadherin 1 , Arc-1 , BCDS1 , CD324 , CDHE , ECAD , LCAM , UVO

Cellular Localization

Cell junction , Cell membrane

Background

This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-E-Cadherin antibody [GT477] used in IHC (Paraffin sections) (IHC-P). GTX629691

GTX629691 IHC-P Image

E-Cadherin antibody [GT477] detects E-Cadherin protein at cell membrane and cytoplasm in mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
E-Cadherin antibody [GT477] (GTX629691) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-E-Cadherin antibody [GT477] used in IHC (Paraffin sections) (IHC-P). GTX629691

GTX629691 IHC-P Image

E-Cadherin antibody [GT477] detects E-Cadherin protein at cell membrane and cytoplasm in rat intestine by immunohistochemical analysis.
Sample: Paraffin-embedded rat intestine.
E-Cadherin antibody [GT477] (GTX629691) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-E-Cadherin antibody [GT477] used in Western Blot (WB). GTX629691

GTX629691 WB Image

Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody [GT477] (GTX629691) diluted at 1:2000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-E-Cadherin antibody [GT477] used in Immunoprecipitation (IP). GTX629691

GTX629691 IP Image

E-adherin antibody [GT477] immunoprecipitates E-adherin protein in IP experiments.
IP samples: MCF-7 whole cell extract
A. Control with 3 μg of preimmune Mouse IgG
B. Immunoprecipitation of E-adherin protein by 3 μg E-adherin antibody [GT477] (GTX629691)
5 % SDS-PAGE
The immunoprecipitated E-adherin protein was detected by E-adherin antibody [GT477] (GTX629691) diluted at 1:500.
[EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent]

Anti-E-Cadherin antibody [GT477] used in Western Blot (WB). GTX629691

GTX629691 WB Image

E-cadherin antibody [GT477] detects E-cadherin protein by Western blot analysis.
A. 30 μg MCF-7 whole cell lysate/extract
B. 30 μg MDA-MB-231 whole cell lysate/extract
5 % SDS-PAGE
E-cadherin antibody [GT477] (GTX629691) dilution: 1:1000

Anti-E-Cadherin antibody [GT477] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629691

GTX629691 ICC/IF Image

E-Cadherin antibody [GT477] detects E-Cadherin protein at cell membrane by immunofluorescent analysis.
Sample: MCF7 cells were fixed in ice-cold MeOH for 5 min.
Green: E-Cadherin protein stained by E-Cadherin antibody [GT477] (GTX629691) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-E-Cadherin antibody [GT477] used in Flow cytometry (FACS). GTX629691

GTX629691 FACS Image

E-Cadherin antibody [GT477] (GTX629691) detects E-Cadherin protein by flow cytometry analysis.
Sample: MCF-7 cell.
Black: Unlabelled sample was used as a control.
Red: E-Cadherin antibody [GT477] (GTX629691) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.

Anti-E-Cadherin antibody [GT477] used in Western Blot (WB). GTX629691

GTX629691 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with E-Cadherin antibody [GT477] (GTX629691) diluted at 1:3000 and competitor's antibody diluted at 1:3000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-E-Cadherin antibody [GT477] used in Western Blot (WB). GTX629691

GTX629691 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody [GT477] (GTX635000) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-E-Cadherin antibody [GT477] used in Western Blot (WB). GTX629691

GTX629691 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody [GT477] (GTX629691) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

REVIEW

E-Cadherin antibody [GT477] Cat No. GTX629691

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Date : Hu Jing-Lan submitted on 07-May-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : Breast cancer cell line
Fixation : 4% PFA in PBS, 25°C, 0.25Hr
Permeabilization /Antigen retrieval : 0.1% Triton X-100 in PBS, 25°C, 0.25Hr
Primary Antibody : Undiluted, 4°C, 24Hr
Date : Anonymous submitted on 21-Jun-2020
Score :
Application Tested : WB
Sample Species : Hu
Sample : ca922 cells
Amount used : 20 μg
Blocking : 10% non-fat milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 24Hr
SDS
PBS.pdf