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E-Cadherin antibody [GT477]

KO/KD Orthogonal
GTX629691_41400_20160816_IHC-P_M_18121411_211.jpg
GTX629691_41400_20160816_IHC-P_R_18121411_781.jpg
GTX629691_41400_20170623_WB_shRNA_watermark_18121411_839.jpg
GTX629691_41400_IP_18121411_740.jpg
GTX629691_41400_WB_18121411_430.jpg
GTX629691_41400_20170524_IFA_18121411_991.jpg
GTX629691_41400_20181207_FACS_19011519_676.jpg

Cat No. GTX629691

Host Mouse
Clonality Monoclonal
Clone Name GT477
Isotype IgG2a
Application WB, ICC/IF, IHC-P, FACS, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
FACS 1:50-1:200
IP 1:100-1:500
Not tested in other applications.

Calculated MW

97 kDa. ( Note )

Positive Control

MCF-7 , MDA-MB-231

Predict Reactivity

Bovine, Dog, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human E-Cadherin. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Arc-1 antibody antibody , CD324 antibody antibody , CDH1 antibody antibody , CDHE antibody antibody , ECAD antibody antibody , LCAM antibody antibody , UVO antibody antibody , cadherin 1 antibody antibody

Cellular Localization

Cell junction , Cell membrane

Background

This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX629691_41400_20160816_IHC-P_M_18121411_211.jpg

GTX629691 IHC-P Image

E-Cadherin antibody [GT477] detects E-Cadherin protein at cell membrane and cytoplasm in mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
E-Cadherin antibody [GT477] (GTX629691) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX629691_41400_20160816_IHC-P_R_18121411_781.jpg

GTX629691 IHC-P Image

E-Cadherin antibody [GT477] detects E-Cadherin protein at cell membrane and cytoplasm in rat intestine by immunohistochemical analysis.
Sample: Paraffin-embedded rat intestine.
E-Cadherin antibody [GT477] (GTX629691) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX629691_41400_20170623_WB_shRNA_watermark_18121411_839.jpg

GTX629691 WB Image

KO/KD

Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody [GT477] (GTX629691) diluted at 1:2000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX629691_41400_IP_18121411_740.jpg

GTX629691 IP Image

E-adherin antibody [GT477] immunoprecipitates E-adherin protein in IP experiments.
IP samples: MCF-7 whole cell extract
A. Control with 3 μg of preimmune Mouse IgG
B. Immunoprecipitation of E-adherin protein by 3 μg E-adherin antibody [GT477] (GTX629691)
5 % SDS-PAGE
The immunoprecipitated E-adherin protein was detected by E-adherin antibody [GT477] (GTX629691) diluted at 1:500.
[EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent]

GTX629691_41400_WB_18121411_430.jpg

GTX629691 WB Image

Orthogonal

E-cadherin antibody [GT477] detects E-cadherin protein by Western blot analysis.
A. 30 μg MCF-7 whole cell lysate/extract
B. 30 μg MDA-MB-231 whole cell lysate/extract
5 % SDS-PAGE
E-cadherin antibody [GT477] (GTX629691) dilution: 1:1000

GTX629691_41400_20170524_IFA_18121411_991.jpg

GTX629691 ICC/IF Image

E-Cadherin antibody [GT477] detects E-Cadherin protein at cell membrane by immunofluorescent analysis.
Sample: MCF7 cells were fixed in ice-cold MeOH for 5 min.
Green: E-Cadherin protein stained by E-Cadherin antibody [GT477] (GTX629691) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX629691_41400_20181207_FACS_19011519_676.jpg

GTX629691 FACS Image

E-Cadherin antibody [GT477] (GTX629691) detects E-Cadherin protein by flow cytometry analysis.
Sample: MCF-7 cell.
Black: Unlabelled sample was used as a control.
Red: E-Cadherin antibody [GT477] (GTX629691) dilution: 1:50.
Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.

SDS
PBS.pdf
Package List Price ($)
$ 319
$ 169