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E-Cadherin antibody [N3C2], Internal

KO/KD
GTX124178_40835_20160602_WB_shRNA_watermark_18121410_380.jpg
GTX124178_40835_20151016_IP_18121410_672.jpg
GTX124178_40835_IFA_18121410_311.jpg
GTX124178_40835_20151015_WB_18121410_543.jpg
GTX124178_40835_20160421_WB_18121410_312.jpg
GTX124178_40835_WB_18121410_976.jpg

Cat No. GTX124178

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IP
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

97 kDa. ( Note )

Positive Control

293T , PC-12 , MCF-7

Predict Reactivity

Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human E-Cadherin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Arc-1 antibody, CD324 antibody, CDHE antibody, ECAD antibody, LCAM antibody, UVO antibody, CDH1 antibody, epithelial cadherin antibody, "cadherin 1, E-cadherin (epithelial) antibody", "calcium-dependent adhesion protein, epithelial antibody", cell-CAM 120/80 antibody, uvomorulin antibody, cadherin-1 antibody, CAM 120/80 antibody, E-Cadherin antibody, "cadherin 1, type 1, E-cadherin (epithelial) antibody"

Cellular Localization

Cell junction , Cell membrane

Background

This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX124178_40835_20160602_WB_shRNA_watermark_18121410_380.jpg

GTX124178 WB Image

KO/KD

Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody [N3C2], Internal (GTX124178) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX124178_40835_20151016_IP_18121410_672.jpg

GTX124178 IP Image

Immunoprecipitation of E-Cadherin protein from MCF-7 whole cell extracts using 5 μg of E-Cadherin antibody [N3C2], Internal (GTX124178).
Western blot analysis was performed using E-Cadherin antibody [N3C2], Internal (GTX124178).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX124178_40835_IFA_18121410_311.jpg

GTX124178 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using E-cadherin(GTX124178) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

GTX124178_40835_20151015_WB_18121410_543.jpg

GTX124178 WB Image

E-Cadherin antibody [N3C2], Internal detects E-Cadherin protein by western blot analysis. Whole cell extracts (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody [N3C2], Internal (GTX124178) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX124178_40835_20160421_WB_18121410_312.jpg

GTX124178 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody [N3C2], Internal (GTX124178) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
That MD-MB-231 showed no E-cadherin expression by WB is based on the publication: PMID: 16495925

GTX124178_40835_WB_18121410_976.jpg

GTX124178 WB Image

Sample (30 µg of whole cell lysate)
A: 293T
5% SDS PAGE
GTX124178 diluted at 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169