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E-Cadherin antibody [RM244]

Anti-E-Cadherin antibody [RM244] used in IHC (Paraffin sections) (IHC-P). GTX33614
Anti-E-Cadherin antibody [RM244] used in Western Blot (WB). GTX33614

Cat No. GTX33614

Host Rabbit
Clonality Monoclonal
Clone Name RM244
Isotype IgG
Application WB, IHC-P
Reactivity Human
Package
100 μl ($279)
APPLICATION

Application Note

Immunohistochemistry (IHC): 1:500-1:1000 dilution, Western Blot (WB): 1:1000-1:2000 dilution.

Calculated MW

97 kDa. ( Note )

Specificity/Sensitivity

This antibody reacts to human E-cadherin (Cadherin-1).
PROPERTIES

Form

Liquid

Buffer

50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

A peptide corresponding to Cadherin-1

Purification

Protein A affinity purified from an animal origin-free culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cadherin 1 , Arc-1 , Bcds1 , Cd324 , Cdhe , Ecad , Lcam , Uvo , Cdh1

Background

This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-E-Cadherin antibody [RM244] used in IHC (Paraffin sections) (IHC-P). GTX33614

GTX33614 IHC-P Image

Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-E-cadherin RM244 at a 1:1000 dilution.

Anti-E-Cadherin antibody [RM244] used in Western Blot (WB). GTX33614

GTX33614 WB Image

Western Blot of MCF-7 cell lysates using Anti-E-cadherin RM244 at a 1:1000 dilution, showed a band of E-cadherin (~120 kDa) expressed in MCF-7 cells.

REFERENCE
There are currently no references for E-Cadherin antibody [RM244] (GTX33614). Be the first to share your publications with this product.
REVIEW
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Package List Price ($)
$ 279