Immunohistochemistry (IHC): 1:500-1:1000 dilution, Western Blot (WB): 1:1000-1:2000 dilution.
This antibody reacts to human E-cadherin (Cadherin-1).
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
A peptide corresponding to Cadherin-1
Protein A affinity purified from an animal origin-free culture supernatant
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Cadherin 1 , Arc-1 , Bcds1 , Cd324 , Cdhe , Ecad , Lcam , Uvo , Cdh1
This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. [provided by RefSeq, Jul 2008]
GTX33614 IHC-P Image
Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-E-cadherin RM244 at a 1:1000 dilution.
GTX33614 WB Image
Western Blot of MCF-7 cell lysates using Anti-E-cadherin RM244 at a 1:1000 dilution, showed a band of E-cadherin (~120 kDa) expressed in MCF-7 cells.