APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 2-4μg/ml |
| 1-2μg/ml for 30 minutes at RT |
|
Note :
IHC-P
Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
|
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human E-Cadherin protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
Arc1 , CD324 , CDH1 , CDHE , ECAD , LCAM , UVO , cadherin 1 , E-Cadherin , Cadherin 1
Cellular Localization
Cell junction,Cell membrane,Endosome,Golgi apparatus,trans-Golgi network
Background
This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16. [provided by RefSeq, Nov 2015]
Database
Research Area
DATA IMAGES
![Anti-E-Cadherin antibody [SPM381] used in IHC (Paraffin sections) (IHC-P). GTX34681](/templates/default/js/lightbox/images/loading.gif "Anti-E-Cadherin antibody [SPM381] used in IHC (Paraffin sections) (IHC-P). GTX34681")
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GTX34681 IHC-P Image
IHC-P analysis of human colon tissue using GTX34681 E-Cadherin antibody [SPM381].
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GTX34681 ICC/IF Image
ICC/IF analysis of MCF-7 cells using GTX34681 E-Cadherin antibody [SPM381].
Green : Primary antibody
Red : nucleus
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REFERENCE
There are currently no references for E-Cadherin antibody [SPM381] (GTX34681). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for E-Cadherin antibody [SPM381] (GTX34681). Be the first to share your experience with this product.
![Anti-E-Cadherin antibody [SPM381] used in IHC (Paraffin sections) (IHC-P). GTX34681](/upload/website/prouct_img/small/GTX34681/GTX34681_IHC-P_1_w_23060801_349.webp "Anti-E-Cadherin antibody [SPM381] used in IHC (Paraffin sections) (IHC-P). GTX34681")
![Anti-E-Cadherin antibody [SPM381] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34681](/upload/website/prouct_img/small/GTX34681/GTX34681_20200115_ICC-IF_1556_w_23060801_565.webp "Anti-E-Cadherin antibody [SPM381] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34681")
![Anti-E-Cadherin antibody [SPM381] used in IHC (Paraffin sections) (IHC-P). GTX34681](/upload/website/prouct_img/normal/GTX34681/GTX34681_IHC-P_1_w_23060801_349.webp "Anti-E-Cadherin antibody [SPM381] used in IHC (Paraffin sections) (IHC-P). GTX34681")
![Anti-E-Cadherin antibody [SPM381] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34681](/upload/website/prouct_img/normal/GTX34681/GTX34681_20200115_ICC-IF_1556_w_23060801_565.webp "Anti-E-Cadherin antibody [SPM381] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34681")