PRODUCT
Summary
E-Cadherin antibody detects E-cadherin protein, which is encoded by the CDH1 gene. E-cadherin is a calcium-dependent cell adhesion protein (predicted molecular weight of 97 kDa) that plays a vital role in cell migration and proliferation. Because E-cadherin is an epithelial cell marker, this antibody is a well-published and widely used reagent to study E-cadherin protein expression during the epithelial-mesenchymal transition (EMT) in various physiological and pathological processes.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:10000 |
1:100-1:1000 |
1:100-1:1000 |
Assay dependent |
1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
MCF-7
Predict Reactivity
Bovine, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS pH7, 1% BSA, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.29 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human E-Cadherin. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10729586
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
cadherin 1 , Arc-1 , BCDS1 , CD324 , CDHE , ECAD , LCAM , UVO
Cellular Localization
Cell junction , Cell membrane
Background
This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX100443 WB Image
Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody (GTX100443) diluted at 1:7000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX100443 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with E-Cadherin antibody (GTX100443) diluted at 1:3000 and competitor's antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX100443 IHC-P Image
E-Cadherin antibody detects E-Cadherin protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat duodenum.E-Cadherin stained by E-Cadherin antibody (GTX100443) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX100443 IHC-P Image
E-cadherin antibody detects E-cadherin protein at membrane on human breast cancer by immunohistochemical analysis. Sample: Paraffin-embedded breast cancer. E-cadherin antibody (GTX100443) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX100443 ICC/IF Image
E-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis. Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: E-Cadherin protein stained by E-Cadherin antibody (GTX100443) diluted at 1:500. Blue: Hoechst 33342 staining.
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GTX100443 IP Image
E-cadherin antibody immunoprecipitates E-cadherin protein in IP experiments. IP samples: MCF-7 whole cell extract A. Control with 3 μg of preimmune Rabbit IgG B. Immunoprecipitation of E-cadherin protein by 3 μg E-cadherin antibody (GTX100443) 5 % SDS-PAGE The immunoprecipitated E-cadherin protein was detected by E-cadherin antibody (GTX100443) diluted at 1:500. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
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GTX100443 IP Image
Immunoprecipitation of E-Cadherin protein from MCF-7 whole cell extracts using 5 μg of E-Cadherin antibody (GTX100443). Western blot analysis was performed using E-Cadherin antibody (GTX100443). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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GTX100443 IHC-P Image
E-Cadherin antibody detects E-Cadherin protein at cell membrane in mouse cervix by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. E-Cadherin antibody (GTX100443) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX100443 IHC-P Image
E-Cadherin antibody detects E-Cadherin protein at cell membrane in rat prostate by immunohistochemical analysis. Sample: Paraffin-embedded rat prostate. E-Cadherin antibody (GTX100443) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX100443 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human ulcerative colitis tissue using E-Cadherin antibody (GTX100443).
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GTX100443 IHC-P Image
E-Cadherin antibody detects E-Cadherin protein at cell membrane in mouse pancreas by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. E-Cadherin antibody (GTX100443) diluted at 1:400.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX100443 ICC/IF Image
E-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: E-Cadherin protein stained by E-Cadherin antibody (GTX100443) diluted at 1:500. Blue: Hoechst 33342 staining.
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GTX100443 IHC-P Image
E-Cadherin antibody detects E-Cadherin protein at cell membrane in rat prostate by immunohistochemical analysis. Sample: Paraffin-embedded rat prostate. E-Cadherin antibody (GTX100443) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX100443 IHC-P Image
E-Cadherin antibody detects E-Cadherin protein at cell membrane in rat intestine by immunohistochemical analysis. Sample: Paraffin-embedded rat intestine. E-Cadherin antibody (GTX100443) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX100443 ICC/IF Image
E-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis.Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: E-Cadherin stained by E-Cadherin antibody (GTX100443) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).Scale bar= 10 μm.
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GTX100443 IHC-P Image
E-Cadherin antibody detects E-Cadherin protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse intestine.E-Cadherin stained by E-Cadherin antibody (GTX100443) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX100443 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody (GTX100443) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX100443 WB Image
Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody (GTX100443) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX100443 IHC-P Image
E-Cadherin antibody detects E-Cadherin protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded dog skin.E-Cadherin stained by E-Cadherin antibody (GTX100443) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX100443 ICC/IF Image
E-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis.Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: E-Cadherin stained by E-Cadherin antibody (GTX100443) diluted at 1:500.
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GTX100443 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody (GTX100443) diluted at 1:7000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX100443 WB Image
Whole cell extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody (GTX100443) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX100443 ICC/IF Image
E-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis.Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: E-Cadherin stained by E-Cadherin antibody (GTX100443) diluted at 1:500.
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GTX100443 IHC-P Image
E-Cadherin antibody detects E-Cadherin protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse stomach.E-Cadherin stained by E-Cadherin antibody (GTX100443) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
REVIEW
E-Cadherin antibody
Cat. No. GTX100443
Rating
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( Average 4.9 based on 16 users reviews)
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Application
Western Blot(WB)
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( Average 4.8 based on 13 users reviews)
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Immunocytochemistry/ Immunofluorescence(ICC/IF)
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( Average 5 based on 1 users reviews)
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IHC (Paraffin sections)(IHC-P)
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( Average 5 based on 2 users reviews)
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Date :
Anonymous submitted on 27-Jan-2022
Application Tested :
WB
Sample Species :
Hu
Sample :
MDA-MB-231 cells, MCF-7 cells
Amount used :
30μg
Blocking :
5% non-fat milk in PBS, 4°C, 1.5Hr
Primary Antibody :
1:3000, 4°C, > 12Hr
Date :
Anonymous submitted on 22-Sep-2021
Application Tested :
ICC/IF
Sample Species :
Hu
Sample :
A549 cells
Fixation :
4% PFA
Permeabilization /Antigen retrieval :
0.2% Triton X-100 in PBS, RT, 0.5Hr
Primary Antibody :
1:100, 4°C, 16Hr
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Front Oncol in 2020.
PMID: 32211324
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Int J Mol Sci in 2019.
PMID: 31426484
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
IHC-P
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal Cell Death Dis in 2020.
PMID: 32080178
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
IHC-P
Sample Species :
Rat
Figure Note :
The data was peer reviewed and published in the journal Evid Based Complement Alternat Med in 2019.
PMID: 31186661
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Rat
Figure Note :
The data was peer reviewed and published in the journal Evid Based Complement Alternat Med in 2019.
PMID: 31186661
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal PLoS One in 2017.
PMID: 28683123
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal PLoS One in 2017.
PMID: 28683123
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Sci Rep in 2017.
PMID: 28195146
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal Front Endocrinol (Lausanne) in 2017.
PMID: 29109696
Date :
Anonymous submitted on 22-Feb-2021
Application Tested :
WB
Sample Species :
Ms
Sample :
colonic mucosa
Amount used :
25 μg
Blocking :
5% fat dry milk in TBST, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 16Hr
Date :
Anonymous submitted on 30-Dec-2020
Application Tested :
WB
Sample Species :
Hu
Sample :
A549 cells
Amount used :
80 μg
Blocking :
1% skim milk, 24°C, 1Hr
Primary Antibody :
1:2000, 24°C, 2Hr
Date :
Anonymous submitted on 21-Jun-2020
Application Tested :
WB
Sample Species :
Hu
Sample :
oecm1 cells
Amount used :
20 μg
Blocking :
10% non-fat milk, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 24Hr
Date :
Anonymous submitted on 26-May-2018
Application Tested :
WB
Sample Species :
Hu
Amount used :
30 μg
Blocking :
5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 16Hr
Lane Description :
Lane 1: hFOB1.19 cells, Lane 2: MG63 cells, Lane 3: U2OS cells
Date :
Anonymous submitted on 24-Apr-2015
Application Tested :
WB
Sample Species :
Hu
Sample :
HT-29 cells
Amount used :
30μg
Blocking :
5% skim milk in TBST, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 16Hr