GeneTex
United States (US)

E-Cadherin antibody

KO/KD Orthogonal
GTX100443_41591_20160602_WB_shRNA_watermark_19011506_490.jpg
GTX100443_41570_20151026_IHC-P_R_19011506_821.jpg
GTX100443_41388_IHC_19011506_379.jpg
GTX100443_42719_20170628_IFA_19011506_461.jpg
GTX100443_42508_20160720_IFA_19011506_143.jpg
GTX100443_41388_IP_19011506_970.jpg
GTX100443_41570_20151016_IP_19011506_795.jpg
GTX100443_42564_20160830_IHC-P_M_19011506_269.jpg
GTX100443_42718_20170205_IHC-P_R_19011506_923.jpg
GTX100443_42718_20170119_WB_19011506_461.jpg
Genetex E Cadherin 443 1 in 20000 400X small  5.4.11_19011720_134.jpg
GTX100443_41591_20160126_IHC-P_M_19011506_982.jpg
GTX100443_42508_20160810_IFA_19011506_805.jpg
GTX100443_42719_20170205_IHC-P_R_19011506_325.jpg
GTX100443_42564_20160830_IHC-P_R_19011506_851.jpg

Cat No. GTX100443

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

97 kDa. ( Note )

Positive Control

MCF-7 , *HT-29

Predict Reactivity

Bovine, Dog, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.6mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human E-Cadherin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Arc-1 antibody, CD324 antibody, CDHE antibody, ECAD antibody, LCAM antibody, UVO antibody, CDH1 antibody, epithelial cadherin antibody, cadherin 1, E-cadherin (epithelial) antibody, calcium-dependent adhesion protein, epithelial antibody, cell-CAM 120/80 antibody, uvomorulin antibody, cadherin-1 antibody, CAM 120/80 antibody, E-Cadherin antibody, cadherin 1, type 1, E-cadherin (epithelial) antibody

Cellular Localization

Cell junction , Cell membrane

Background

This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100443_41591_20160602_WB_shRNA_watermark_19011506_490.jpg

GTX100443 WB Image

KO/KD

Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody (GTX100443) diluted at 1:7000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100443_41570_20151026_IHC-P_R_19011506_821.jpg

GTX100443 IHC-P Image

E-Cadherin antibody detects E-Cadherin protein at cell membrane and cytoplasm in rat duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded rat duodenum.
E-Cadherin antibody (GTX100443) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100443_41388_IHC_19011506_379.jpg

GTX100443 IHC-P Image

E-cadherin antibody detects E-cadherin protein at membrane on human breast cancer by immunohistochemical analysis.
Sample: Paraffin-embedded breast cancer.
E-cadherin antibody (GTX100443) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100443_42719_20170628_IFA_19011506_461.jpg

GTX100443 ICC/IF Image

E-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis.
Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: E-Cadherin protein stained by E-Cadherin antibody (GTX100443) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX100443_42508_20160720_IFA_19011506_143.jpg

GTX100443 ICC/IF Image

E-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis.
Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: E-Cadherin protein stained by E-Cadherin antibody (GTX100443) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX100443_41388_IP_19011506_970.jpg

GTX100443 IP Image

E-cadherin antibody immunoprecipitates E-cadherin protein in IP experiments.
IP samples: MCF-7 whole cell extract
A. Control with 3 μg of preimmune Rabbit IgG
B. Immunoprecipitation of E-cadherin protein by 3 μg E-cadherin antibody (GTX100443)
5 % SDS-PAGE
The immunoprecipitated E-cadherin protein was detected by E-cadherin antibody (GTX100443) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

GTX100443_41570_20151016_IP_19011506_795.jpg

GTX100443 IP Image

Immunoprecipitation of E-Cadherin protein from MCF-7 whole cell extracts using 5 μg of E-Cadherin antibody (GTX100443).
Western blot analysis was performed using E-Cadherin antibody (GTX100443).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX100443_42564_20160830_IHC-P_M_19011506_269.jpg

GTX100443 IHC-P Image

E-Cadherin antibody detects E-Cadherin protein at cell membrane in mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
E-Cadherin antibody (GTX100443) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100443_42718_20170205_IHC-P_R_19011506_923.jpg

GTX100443 IHC-P Image

E-Cadherin antibody detects E-Cadherin protein at cell membrane in rat prostate by immunohistochemical analysis.
Sample: Paraffin-embedded rat prostate.
E-Cadherin antibody (GTX100443) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100443_42718_20170119_WB_19011506_461.jpg

GTX100443 WB Image

Orthogonal

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody (GTX100443) diluted at 1:2000.
The observed M.W. is based on the publication: PMID: 20356845

Genetex E Cadherin 443 1 in 20000 400X small  5.4.11_19011720_134.jpg

GTX100443 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human ulcerative colitis tissue using E-Cadherin antibody (GTX100443).

GTX100443_41591_20160126_IHC-P_M_19011506_982.jpg

GTX100443 IHC-P Image

E-Cadherin antibody detects E-Cadherin protein at cell membrane in mouse pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
E-Cadherin antibody (GTX100443) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100443_42508_20160810_IFA_19011506_805.jpg

GTX100443 ICC/IF Image

E-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: E-Cadherin protein stained by E-Cadherin antibody (GTX100443) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX100443_42719_20170205_IHC-P_R_19011506_325.jpg

GTX100443 IHC-P Image

E-Cadherin antibody detects E-Cadherin protein at cell membrane in rat prostate by immunohistochemical analysis.
Sample: Paraffin-embedded rat prostate.
E-Cadherin antibody (GTX100443) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100443_42564_20160830_IHC-P_R_19011506_851.jpg

GTX100443 IHC-P Image

E-Cadherin antibody detects E-Cadherin protein at cell membrane in rat intestine by immunohistochemical analysis.
Sample: Paraffin-embedded rat intestine.
E-Cadherin antibody (GTX100443) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
REVIEW

E-Cadherin antibody Cat No. GTX100443

Rating ( Average 4.5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 4.5 based on 2 users reviews)
Date : Anonymous submitted on 26-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
Lane Description : Lane 1: hFOB1.19 cells, Lane 2: MG63 cells, Lane 3: U2OS cells
Date : Anonymous submitted on 24-Apr-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : HT-29 cells
Amount used : 30μg
Blocking : 5% skim milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169