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E-Cadherin antibody

Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443
Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443
Anti-E-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX100443
Anti-E-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX100443
Anti-E-Cadherin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100443
Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443
Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443
Anti-E-Cadherin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100443
Anti-E-Cadherin antibody used in Immunoprecipitation (IP). GTX100443
Anti-E-Cadherin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100443
Anti-E-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX100443
Anti-E-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX100443
Anti-E-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX100443
Anti-E-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX100443
Anti-E-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX100443
Anti-E-Cadherin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100443
Anti-E-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX100443
Anti-E-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX100443
Anti-E-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX100443
Anti-E-Cadherin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100443
Anti-E-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX100443
Anti-E-Cadherin antibody used in Immunoprecipitation (IP). GTX100443
Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443
Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443
Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443
Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443
Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443
Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443
Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443
Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443
Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443
Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443
Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443
Anti-E-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX100443
Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443
Anti-E-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX100443
Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443

Cat. No. GTX100443

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Wm, IP, PLA

Reactivity

Human, Mouse, Rat, Zebrafish, Dog
Package
100 μl ($399),
25 μl ($169)
PRODUCT

Summary

E-Cadherin antibody detects E-cadherin protein, which is encoded by the CDH1 gene. E-cadherin is a calcium-dependent cell adhesion protein (predicted molecular weight of 97 kDa) that plays a vital role in cell migration and proliferation. Because E-cadherin is an epithelial cell marker, this antibody is a well-published and widely used reagent to study E-cadherin protein expression during the epithelial-mesenchymal transition (EMT) in various physiological and pathological processes.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
IP 1:100-1:500
PLA Assay dependent
Not tested in other applications.

Calculated MW

97 kDa. ( Note )

Positive Control

MCF-7

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.14 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human E-Cadherin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10729586

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cadherin 1 , Arc-1 , BCDS1 , CD324 , CDHE , ECAD , LCAM , UVO

Cellular Localization

Cell junction , Cell membrane

Background

This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443

GTX100443 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with E-Cadherin antibody (GTX100443) diluted at 1:3000 and competitor's antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443

GTX100443 WB Image

Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody (GTX100443) diluted at 1:7000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-E-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX100443

GTX100443 IHC-P Image

E-Cadherin antibody detects E-Cadherin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat duodenum.
E-Cadherin stained by E-Cadherin antibody (GTX100443) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-E-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX100443

GTX100443 IHC-P Image

E-Cadherin antibody detects E-Cadherin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog skin.
E-Cadherin stained by E-Cadherin antibody (GTX100443) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-E-Cadherin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100443

GTX100443 ICC/IF Image

E-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: E-Cadherin stained by E-Cadherin antibody (GTX100443) diluted at 1:500.

Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443

GTX100443 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody (GTX100443) diluted at 1:7000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443

GTX100443 WB Image

Whole cell extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody (GTX100443) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-E-Cadherin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100443

GTX100443 ICC/IF Image

E-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: E-Cadherin stained by E-Cadherin antibody (GTX100443) diluted at 1:500.

Anti-E-Cadherin antibody used in Immunoprecipitation (IP). GTX100443

GTX100443 IP Image

E-cadherin antibody immunoprecipitates E-cadherin protein in IP experiments.
IP samples: MCF-7 whole cell extract
A. Control with 3 μg of preimmune Rabbit IgG
B. Immunoprecipitation of E-cadherin protein by 3 μg E-cadherin antibody (GTX100443)
5 % SDS-PAGE
The immunoprecipitated E-cadherin protein was detected by E-cadherin antibody (GTX100443) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Anti-E-Cadherin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100443

GTX100443 ICC/IF Image

E-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis.
Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: E-Cadherin stained by E-Cadherin antibody (GTX100443) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

Anti-E-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX100443

GTX100443 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human ulcerative colitis tissue using E-Cadherin antibody (GTX100443).

Anti-E-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX100443

GTX100443 IHC-P Image

E-Cadherin antibody detects E-Cadherin protein at cell membrane in rat prostate by immunohistochemical analysis.
Sample: Paraffin-embedded rat prostate.
E-Cadherin antibody (GTX100443) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-E-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX100443

GTX100443 IHC-P Image

E-cadherin antibody detects E-cadherin protein at membrane on human breast cancer by immunohistochemical analysis.
Sample: Paraffin-embedded breast cancer.
E-cadherin antibody (GTX100443) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-E-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX100443

GTX100443 IHC-P Image

E-Cadherin antibody detects E-Cadherin protein at cell membrane in rat prostate by immunohistochemical analysis.
Sample: Paraffin-embedded rat prostate.
E-Cadherin antibody (GTX100443) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-E-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX100443

GTX100443 IHC-P Image

E-Cadherin antibody detects E-Cadherin protein at cell membrane in mouse pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
E-Cadherin antibody (GTX100443) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-E-Cadherin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100443

GTX100443 ICC/IF Image

E-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: E-Cadherin protein stained by E-Cadherin antibody (GTX100443) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-E-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX100443

GTX100443 IHC-P Image

E-Cadherin antibody detects E-Cadherin protein at cell membrane in rat intestine by immunohistochemical analysis.
Sample: Paraffin-embedded rat intestine.
E-Cadherin antibody (GTX100443) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-E-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX100443

GTX100443 IHC-P Image

E-Cadherin antibody detects E-Cadherin protein at cell membrane in mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
E-Cadherin antibody (GTX100443) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-E-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX100443

GTX100443 IHC-P Image

E-Cadherin antibody detects E-Cadherin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
E-Cadherin stained by E-Cadherin antibody (GTX100443) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-E-Cadherin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100443

GTX100443 ICC/IF Image

E-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis.
Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: E-Cadherin protein stained by E-Cadherin antibody (GTX100443) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-E-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX100443

GTX100443 IHC-P Image

E-Cadherin antibody detects E-Cadherin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
E-Cadherin stained by E-Cadherin antibody (GTX100443) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-E-Cadherin antibody used in Immunoprecipitation (IP). GTX100443

GTX100443 IP Image

Immunoprecipitation of E-Cadherin protein from MCF-7 whole cell extracts using 5 μg of E-Cadherin antibody (GTX100443).
Western blot analysis was performed using E-Cadherin antibody (GTX100443).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443

GTX100443 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody (GTX100443) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443

GTX100443 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody (GTX100443) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443

GTX100443 WB Image

The data was published in the 2022 in Front Pharmacol. PMID: 35517780

Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443

GTX100443 WB Image

The data was published in the journal Front Oncol in 2020.PMID: 32211324

Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443

GTX100443 WB Image

The data was published in the 2022 in Front Pharmacol. PMID: 35517780

Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443

GTX100443 WB Image

The data was published in the 2022 in Front Pharmacol. PMID: 35517780

Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443

GTX100443 WB Image

The data was published in the 2022 in Front Pharmacol. PMID: 35517780

Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443

GTX100443 WB Image

The data was published in the journal PLoS One in 2017. PMID: 28683123

Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443

GTX100443 WB Image

The data was published in the journal PLoS One in 2017. PMID: 28683123

Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443

GTX100443 WB Image

The data was published in the journal Sci Rep in 2017. PMID: 28195146

Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443

GTX100443 WB Image

The data was published in the journal Front Endocrinol (Lausanne) in 2017. PMID: 29109696

Anti-E-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX100443

GTX100443 IHC-P Image

The data was published in the journal Cell Death Dis in 2020.PMID: 32080178

Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443

GTX100443 WB Image

The data was published in the journal Int J Mol Sci in 2019.PMID: 31426484

Anti-E-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX100443

GTX100443 IHC-P Image

The data was published in the journal Evid Based Complement Alternat Med in 2019. PMID: 31186661

Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443

GTX100443 WB Image

The data was published in the journal Evid Based Complement Alternat Med in 2019. PMID: 31186661

REFERENCE

REVIEW

E-Cadherin antibody Cat. No. GTX100443

Rating ( Average 4.8 based on 9 users reviews)
Application
Western Blot(WB) ( Average 4.7 based on 6 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 02-May-2024
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : Mouse liver tissue
Fixation : 4% PFA in PBS
Permeabilization /Antigen retrieval : Citrate buffer, pH 6.0, 0.25Hr
Primary Antibody : 1:1000, 4°C, 16-18Hr
Date : Anonymous submitted on 10-Oct-2023
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : Mouse Brain tissue
Blocking : 0.5% skim milk in PBST
Fixation : 4% PFA
Permeabilization /Antigen retrieval : PBS-T / Microwave heating in citrate buffer
Primary Antibody : 1:1000, 4°C, 18Hr
Date : Anonymous submitted on 27-Jan-2022
Score :
Application Tested : WB
Sample Species : Hu
Sample : MDA-MB-231 cells, MCF-7 cells
Amount used : 30μg
Blocking : 5% non-fat milk in PBS, 4°C, 1.5Hr
Primary Antibody : 1:3000, 4°C, > 12Hr
Date : Anonymous submitted on 22-Sep-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : A549 cells
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.2% Triton X-100 in PBS, RT, 0.5Hr
Primary Antibody : 1:100, 4°C, 16Hr
Date : Anonymous submitted on 22-Feb-2021
Score :
Application Tested : WB
Sample Species : Ms
Sample : colonic mucosa
Amount used : 25 μg
Blocking : 5% fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Date : Anonymous submitted on 30-Dec-2020
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells
Amount used : 80 μg
Blocking : 1% skim milk, 24°C, 1Hr
Primary Antibody : 1:2000, 24°C, 2Hr
Date : Anonymous submitted on 21-Jun-2020
Score :
Application Tested : WB
Sample Species : Hu
Sample : oecm1 cells
Amount used : 20 μg
Blocking : 10% non-fat milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 24Hr
Date : Anonymous submitted on 26-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description : Lane 1: hFOB1.19 cells, Lane 2: MG63 cells, Lane 3: U2OS cells
Date : Anonymous submitted on 24-Apr-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : HT-29 cells
Amount used : 30μg
Blocking : 5% skim milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Application Reference
Wu CH et al. Cancers (Basel) 2023; 15 (5)
Application : WB
Reactivity : Human
Ting-Ting Yen et al. Sci Rep 2021; 11 (1):21737
Application : WB, IHC-P
Reactivity : Human, Mouse
Wang J et al. Onco Targets Ther 2020; 13:9333-9348
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169