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E-Cadherin antibody

KO/KD
Anti-E-Cadherin antibody used in Western Blot (WB). GTX124198
Anti-E-Cadherin antibody used in Western Blot (WB). GTX124198

Cat. No. GTX124198

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all E-Cadherin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

97 kDa. ( Note )

Positive Control

MCF-7

Predict Reactivity

Mouse, Rat, Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human E-Cadherin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885599

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cadherin 1 , Arc-1 , BCDS1 , CD324 , CDHE , ECAD , LCAM , UVO

Cellular Localization

Cell junction , Cell membrane

Background

This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-E-Cadherin antibody used in Western Blot (WB). GTX124198

GTX124198 WB Image

KO/KD

Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody (GTX124198) diluted at 1:1500.

Anti-E-Cadherin antibody used in Western Blot (WB). GTX124198

GTX124198 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody (GTX124198) diluted at 1:500.
The observed M.W. is based on the publication: PMID: 16495925

REFERENCE

Application Reference

M Wang et al. Phytomedicine 2024; 127 : 155391 Arenobufagin inhibits lung metastasis of colorectal cancer by targeting c-MYC/Nrf2 axis
Tonglei Chen et al. J Cancer 2021; 12 (2) : 467-473 MUC 15 Promotes Osteosarcoma Cell Proliferation, Migration and Invasion through Livin, MMP-2/MMP-9 and Wnt/β-Catenin Signal Pathway.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169