GeneTex
  • Country / Location Selection

United States (US)

E-Cadherin (phospho Ser838/840) antibody

Anti-E-Cadherin (phospho Ser838/840) antibody used in Immunoprecipitation (IP). GTX130427
Anti-E-Cadherin (phospho Ser838/840) antibody used in Western Blot (WB). GTX130427
Anti-E-Cadherin (phospho Ser838/840) antibody used in Western Blot (WB). GTX130427
Anti-E-Cadherin (phospho Ser838/840) antibody used in Western Blot (WB). GTX130427

Cat No. GTX130427

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IP 1:72-1:500
Not tested in other applications.

Calculated MW

97 kDa. ( Note )

Positive Control

C8D30 , NIH-3T3 , C2C12 , MCF-7 , MCF-7 CIP , PI , Rat brain

Predict Reactivity

Dog, Pig, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide corresponding to residues around human E-Cadherin (phospho Ser838/840). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886263

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cadherin 1 , Arc-1 , BCDS1 , CD324 , CDHE , ECAD , LCAM , UVO

Cellular Localization

Cell junction , Cell membrane

Background

This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-E-Cadherin (phospho Ser838/840) antibody used in Immunoprecipitation (IP). GTX130427

GTX130427 IP Image

Immunoprecipitation of E-Cadherin (phospho Ser838/840) protein from MCF-7 whole cell extracts using 5 μg of E-Cadherin (phospho Ser838/840) antibody (GTX130427).
Western blot analysis was performed using E-Cadherin (phospho Ser838/840) antibody (GTX130427).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-E-Cadherin (phospho Ser838/840) antibody used in Western Blot (WB). GTX130427

GTX130427 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin (phospho Ser838/840) antibody (GTX130427) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-E-Cadherin (phospho Ser838/840) antibody used in Western Blot (WB). GTX130427

GTX130427 WB Image

E-Cadherin (phospho Ser838/840) antibody detects E-Cadherin (phospho Ser838/840) protein by western blot analysis. MCF-7 whole cell extract (30 μg) with the indicated treatments were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin (phospho Ser838/840) antibody (GTX130427) at a dilution of 1:10000.
PI : Phosphate inhibitors (pervanadate) CIP : Calf Intestinal Alkaline Phosophatase

Anti-E-Cadherin (phospho Ser838/840) antibody used in Western Blot (WB). GTX130427

GTX130427 WB Image

E-Cadherin (phospho Ser838/840) antibody detects E-Cadherin (phospho Ser838/840) protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with E-Cadherin (phospho Ser838/840) antibody (GTX130427) diluted at 1:10000.

REFERENCE

There are currently no references for E-Cadherin (phospho Ser838/840) antibody (GTX130427). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for E-Cadherin (phospho Ser838/840) antibody (GTX130427). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf