E-Cadherin (phospho Ser838/Ser840) antibody [HL3201]

Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with E-Cadherin (phospho Ser838/Ser840) antibody [HL3201] (GTX640841) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) mouse tissue extract (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with E-Cadherin (phospho Ser838/Ser840) antibody [HL3201] (GTX640841) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with E-Cadherin (phospho Ser838/Ser840) antibody [HL3201] (GTX640841) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Specificity test was performed by coating the plate with (CDH1 peptide, CDH1 (phospho Ser838) peptide, CDH1 (phospho Ser840) peptide, and CDH1 (phospho Ser838/Ser840) peptide) (1000- ng/mL). Coated peptides were probed with E-Cadherin (phospho Ser838/Ser840) antibody [HL3201] (GTX640841) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.