Detection LT in culture medium and crude extract of ETEC cells by western blotting, using anti-LT antibody Samples 1. SDS-PAGE of the culture medium of ETEC, CBB stained. 2. SDS-PAGE of the crude extract of ETEC cells, CBB stained. 3. Western Blotting of the culture medium 4 .Westerns blotting of crude extract of ETEC cells. * Indicates a non-specific protein band.

Immunopprecipitation of LT from culture medium of ETEC by using anti-LT antibody H chain and L chain indicate heavy chain and light chain of IgG, respectively.