GeneTex
United States (US)

E. coli LT toxin antibody

GTX64109_20170328_IP_18121411_188.jpg
GTX64109_20170328_WB_18121411_425.jpg

Cat No. GTX64109

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP
Reactivity E. coli
APPLICATION

Application Note

Western blotting (1:2,000-1:10,000 dilution)
PROPERTIES

Form

Liquid

Buffer

Rabbit antiserum added with 0.09%sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

E. coli

Immunogen

Initial immunization with LT toxoid and booster with LT toxin.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
GTX64109_20170328_IP_18121411_188.jpg

GTX64109 IP Image

Immunopprecipitation of LT from culture medium of ETEC by using anti-LT antibody H chain and L chain indicate heavy chain and light chain of IgG, respectively.

GTX64109_20170328_WB_18121411_425.jpg

GTX64109 WB Image

Detection LT in culture medium and crude extract of ETEC cells by western blotting, using anti-LT antibody Samples 1. SDS-PAGE of the culture medium of ETEC, CBB stained. 2. SDS-PAGE of the crude extract of ETEC cells, CBB stained. 3. Western Blotting of the culture medium 4 .Westerns blotting of crude extract of ETEC cells. * Indicates a non-specific protein band.

Package List Price ($)
$ 349