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United States (US)

E. coli antibody (Biotin)

Cat No. GTX40640

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application ELISA, Functional Assay
Reactivity E. coli
Package
1 ml ($339)
APPLICATION

Application Note

Avidin and streptavidin amplification systems for ELISA: Use at an assay dependent dilution. ICC/IF: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

Specificity/Sensitivity

All "O" and "K" antigenic serotypes of Escherichia coli. Will remove E. coli proteins from recombinant preparations.
PROPERTIES

Form

Liquid

Buffer

Preservative: 0.1% Sodium Azide. Constituents: 0.01M PBS. pH 7.2

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

4 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

E. coli

Immunogen

Tissue/cell preparation (Mixture of E. coli serotypes).

Purification

Protein A purified
Protein A chromatography.

Conjugation

Biotin

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Cellular Localization

Whole cell

Background

Escherichia coli is a gram negative bacillus that belongs to a larger group of Enterobacteriae - bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins.

Research Area

REFERENCE
REVIEW
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Package List Price ($)
$ 339