ELISA: 1/2000 by ELISA vs serotypes O157:H7, O20, O125, O55, O111 & K12. IHC: Use at an assay dependent dilution. Immunblotting: Use at an assay dependent dilution. Fluorescence miscroscopy: Use at an assay dependent dilution. GTX13626 may be used in place of neat antiserum in almost any appropriate antibody-based technique. Will remove E. coli proteins from recombinant preparations. GTX13626 is suitable for conjugation purposes. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
GTX13626 reacts with all ""O"" and ""K"" antigenic serotypes of Escherichia coli.
Preservative: 0.1% Sodium Azide; Constituents: 0.01M PBS. pH 7.2
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Batch dependent (Please refer to the vial label for the specific concentration.)
Mixtures of all antigenic serotypes.
This product consists of the purified IgG fraction of the above antiserum prepared by methods developed to a purity of >95%.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Escherichia coli is a gram negative bacillus that belongs to a larger group of Enterobacteriae - bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins.