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E2F1 antibody [10D1 2B5]

Anti-E2F1 antibody [10D1 2B5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70161

Cat No. GTX70161

Host

Mouse

Clonality

Monoclonal

Clone Name

10D1 2B5

Isotype

IgG2b

Application

WB, ICC/IF, IP

Reactivity

Human
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 5 μg/ml
ICC/IF Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

E2F-1 transfected Soas2 cells

PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline, pH 7.4, containing no preservatives

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The region encoding the DP-1 binding site (amino acids 223-332) of E2F-1 expressed in E. coli.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

E2F Transcription Factor 1 , E2F-1 , Rbap1 , Rbbp3 , Rbp3 , E2F1

Cellular Localization

Nuclear

Background

E2F-1-10D1 recognizes E2F-1 that was identified as a cellular protein with DNA binding activity associated with the adenovirus E2 gene promoter. The original member of the E2F family of transcription factors, now consisting of five, was identified through its association with the retinoblastoma protein, which controls its activity. E2F-1 is cell cycle regulated with very low levels in early G1 then increasing levels as cells move from G1 to S with highest levels of protein at the G1/S boundary, which is consistent with its role in S-phase entry.

Database

Research Area

DATA IMAGES

Anti-E2F1 antibody [10D1 2B5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70161

GTX70161 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using E2F1(GTX70161) antibody at 1:100 dilution.

REFERENCE

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REVIEW

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Package List Price ($)
$ 329