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E2F1 antibody [16G7]

Anti-E2F1 antibody [16G7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70163
Anti-E2F1 antibody [16G7] used in Western Blot (WB). GTX70163

Cat. No. GTX70163

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 16G7

Isotype

 IgG1

Application

 WB, ICC/IF, IP

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all E2F1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

Jurkat

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.86 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The region encoding the DNA binding domain (amino acids 244-264) of E2F-1 expressed in E. coli.

Purification

Affinity purified by Protein G.
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_370184

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

E2F transcription factor 1 , E2F-1 , RBAP1 , RBBP3 , RBP3

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-E2F1 antibody [16G7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70163

GTX70163 ICC/IF Image

E2F1 antibody [16G7] detects E2F1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: E2F1 protein stained by E2F1 antibody [16G7] (GTX70163) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
Anti-E2F1 antibody [16G7] used in Western Blot (WB). GTX70163

GTX70163 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with E2F1 antibody [16G7] (GTX70163) diluted at 1:1000. The signal was developed with Trident ECL plus-Enhanced.

REFERENCE

Application Reference

Wang LT et al. Cancer Lett 2021; 520 : 160-171 Phosphorylation of intestine-specific homeobox by ERK1 modulates oncogenic activity and sorafenib resistance.
Application : WB
Reactivity : Mouse
Chen HL et al. Anticancer Res 2020; 40 (9) : 4947-4960 Effects of Sclareol Against Small Cell Lung Carcinoma and the Related Mechanism: In Vitro and In Vivo Studies.
Application : WB
Reactivity : Human
Zhong WB et al. Int J Mol Sci 2018; 19 (7) A Synergistic Anti-Cancer Effect of Troglitazone and * in a Human Anaplastic Thyroid Cancer Cell Line and in a Mouse Xenograft Model.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations