E2F1 antibody [17E2]

Cat. No. GTX70154

Host	Mouse
Clonality	Monoclonal
Clone Name	17E2
Isotype	IgG1
Application	WB, IHC-P, IP
Reactivity	Human, Mouse
Package	100 μl ($399)

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Datasheet
Document

View product citations for antibody GTX70154 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1 - 5 μg/ml
IHC-P	Assay dependent
IP	Assay dependent

Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

E2F-1 transfected Soas2 cells

Product Note

This antibody recognizes E2F-1 that was identified as a cellular protein with DNA binding activity associated with the adenovirus E2 gene promoter.

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The transactivation and retinoblastoma protein binding domain (amino acids 332-390) of E2F-1 expressed in E. coli.

Purification

Protein G affinity purified.
From hybridoma culture supernatant.

Conjugation

Unconjugated

RRID

AB_370180

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

E2F transcription factor 1 , E2F-1 , RBAP1 , RBBP3 , RBP3

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. [provided by RefSeq, Jul 2008]

Database

Gene ID: 1869 E2F1
UniProt: Q01094 E2F1

Research Area

DATA IMAGES

GTX70154 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with E2F1 antibody [17E2] (GTX70154) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX70154 IHC-P Image

E2F1 antibody [17E2] detects E2F1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
E2F1 stained by E2F1 antibody [17E2] (GTX70154) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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SDS
PBS.pdf

Package

List Price ($)

100 μl

$ 399

Qty