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E2F1 antibody [18E10]

Anti-E2F1 antibody [18E10] used in IHC (Paraffin sections) (IHC-P). GTX70165
Anti-E2F1 antibody [18E10] used in Western Blot (WB). GTX70165

Cat. No. GTX70165

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 18E10

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all E2F1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

E2F-1 transfected Soas2 cells

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The region encoding the DNA binding domain (amino acids 244-264) of E2F-1 expressed in E. coli.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_370186

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

E2F transcription factor 1 , E2F-1 , RBAP1 , RBBP3 , RBP3

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-E2F1 antibody [18E10] used in IHC (Paraffin sections) (IHC-P). GTX70165

GTX70165 IHC-P Image

E2F1 antibody [18E10] detects E2F1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
E2F1 stained by E2F1 antibody [18E10] (GTX70165) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-E2F1 antibody [18E10] used in Western Blot (WB). GTX70165

GTX70165 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
10% SDS PAGE
GTX70165 diluted at 1:1000

REFERENCE

Application Reference

Malhotra S et al. PLoS One 2011; 6 (5) : e20293 A tri-marker proliferation index predicts biochemical recurrence after surgery for prostate cancer.
Application : IHC-P
Reactivity : Human
Liu X et al. J Biol Chem 2006; 281 (1) : 578-86 Structure of the human Papillomavirus E7 oncoprotein and its mechanism for inactivation of the retinoblastoma tumor suppressor.
Application : WB
Submit a Reference

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SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations