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E2F1 antibody [4G8]

Anti-E2F1 antibody [4G8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66839
Anti-E2F1 antibody [4G8] used in Western Blot (WB). GTX66839
Anti-E2F1 antibody [4G8] used in Immunoprecipitation (IP). GTX66839

Cat. No. GTX66839

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4G8

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($459)
See all E2F1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500
ICC/IF 1:50-1:200
IP Assay dependent
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.2% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant human E2F-1 protein fragments expressed in E.coli.

Purification

Purified by antigen-affinity chromatography

Purity

> 95% (by SDS-PAGE)

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

E2F transcription factor 1 , E2F-1 , RBAP1 , RBBP3 , RBP3

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-E2F1 antibody [4G8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66839

GTX66839 ICC/IF Image

ICC/IF analysis of 4% PFA-fixed HeLa cells using GTX66839 E2F1 antibody [4G8].
Blue : DAPI
Dilution : 1:100

Anti-E2F1 antibody [4G8] used in Western Blot (WB). GTX66839

GTX66839 WB Image

WB analysis of C6, Raw264.7, and Hela cell lysates using GTX66839 E2F1 antibody [4G8].
Dilution : 1:500

Anti-E2F1 antibody [4G8] used in Immunoprecipitation (IP). GTX66839

GTX66839 IP Image

IP analysis of HeLa whole cell lysate using GTX66839 E2F1 antibody [4G8].

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 459