Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with E2F1 antibody [6B5] (GTX70167) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with E2F1 antibody [6B5] (GTX70167) diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.