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E2F1 antibody [6B5]

Overexpression
Anti-E2F1 antibody [6B5] used in Western Blot (WB). GTX70167
Anti-E2F1 antibody [6B5] used in Western Blot (WB). GTX70167

Cat. No. GTX70167

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 6B5

Isotype

 IgG1

Application

 WB, IP

Reactivity

 Human
Package
100 μl ($399)
See all E2F1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IP Assay dependent
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

MCF-7 , E2F1-transfected 293T

Product Note

This antibody recognizes E2F-1 that was identified as a cellular protein with DNA binding activity associated with the adenovirus E2 gene promoter.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.29 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The region encoding the DP-1 binding site (amino acids 223-332) of E2F-1 expressed in E. coli.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_370188

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

E2F transcription factor 1 , E2F-1 , RBAP1 , RBBP3 , RBP3

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-E2F1 antibody [6B5] used in Western Blot (WB). GTX70167

GTX70167 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with E2F1 antibody [6B5] (GTX70167) diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.

Anti-E2F1 antibody [6B5] used in Western Blot (WB). GTX70167

GTX70167 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with E2F1 antibody [6B5] (GTX70167) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations