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E2F1 antibody [8G9]

Anti-E2F1 antibody [8G9] used in Flow cytometry (FACS). GTX60615
Anti-E2F1 antibody [8G9] used in IHC (Paraffin sections) (IHC-P). GTX60615
Anti-E2F1 antibody [8G9] used in ELISA (ELISA). GTX60615
Anti-E2F1 antibody [8G9] used in Western Blot (WB). GTX60615
Anti-E2F1 antibody [8G9] used in IHC (Paraffin sections) (IHC-P). GTX60615

Cat. No. GTX60615

Host

Mouse

Clonality

Monoclonal

Clone Name

8G9

Isotype

IgG1

Application

WB, IHC-P, FACS, ELISA

Reactivity

Human, Mouse
Package
100 μg ($409)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human E2F1 (AA: 69-223) expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

E2F transcription factor 1 , E2F-1 , RBAP1 , RBBP3 , RBP3

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-E2F1 antibody [8G9] used in Flow cytometry (FACS). GTX60615

GTX60615 FACS Image

FACS analysis of HeLa cells using GTX60615 E2F1 antibody [8G9].
Green : E2F1
Red : negative control

Anti-E2F1 antibody [8G9] used in IHC (Paraffin sections) (IHC-P). GTX60615

GTX60615 IHC-P Image

IHC-P analysis of rectum cancer tissue using GTX60615 E2F1 antibody [8G9].

Anti-E2F1 antibody [8G9] used in ELISA (ELISA). GTX60615

GTX60615 ELISA Image

ELISA analysis of antigen using GTX60615 E2F1 antibody [8G9].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-E2F1 antibody [8G9] used in Western Blot (WB). GTX60615

GTX60615 WB Image

WB analysis of HeLa (1), SK-N-SH (2), and NIH3T3 (3) cell lysate using GTX60615 E2F1 antibody [8G9].

Anti-E2F1 antibody [8G9] used in IHC (Paraffin sections) (IHC-P). GTX60615

GTX60615 IHC-P Image

IHC-P analysis of esophageal cancer tissue using GTX60615 E2F1 antibody [8G9].

REFERENCE

There are currently no references for E2F1 antibody [8G9] (GTX60615). Be the first to share your publications with this product.

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409