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E2F1 antibody [KH95]

Anti-E2F1 antibody [KH95] used in IHC (Paraffin sections) (IHC-P). GTX24070

Cat. No. GTX24070

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 KH95

Isotype

 IgG2a

Application

 WB, IHC-P, IP, ChIP assay

Reactivity

 Human
Package
500 μl ($509)
See all E2F1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P 1:25‐1:50
IP Assay dependent
ChIP assay Assay dependent

Note :

IHC-P
Recommendations for antigen retrieval : EDTA buffer pH 8.0 at room temperature for 30 mins.
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Antigen Species

Human

Immunogen

BALB/C mice were immunized with a recombinant human E2F-1 protein.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_370171

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

E2F transcription factor 1 , E2F-1 , RBAP1 , RBBP3 , RBP3

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-E2F1 antibody [KH95] used in IHC (Paraffin sections) (IHC-P). GTX24070

GTX24070 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX24070 E2F1 antibody [KH95].

REFERENCE

Application Reference

Tetikkurt US et al. Appl Immunohistochem Mol Morphol 2010; 18 (4) : 338-43 Predicting malignant potential of gastrointestinal stromal tumors: Role of p16 and E2F1 expression.

Clone Reference

Chuangui Wang et al. J Biol Chem 2007; 282 (41):29902-9 Regulation of E2F1 function by the nuclear corepressor KAP1
Application : WB, IP, ChIP assay
Reactivity : Human
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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 509