GeneTex
United States (US)

E2F1 antibody [N1N3]

GTX101235_39812_20181221_WB_B_19011519_767.jpg
GTX101235_WB_19011506_801.jpg
GTX101235_39812_IP_19011506_492.jpg
GTX101235_39812_20151216_IFA_19011506_570.jpg
GTX101235_39812_20151231_WB_M_19011506_160.jpg

Cat No. GTX101235

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IP
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , NIH-3T3 , E2F1-transfected 293T

Predict Reactivity

Rat, Bovine, Chicken(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human E2F1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

E2F Transcription Factor 1,E2F-1,Rbap1,Rbbp3,Rbp3,E2F1

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101235_39812_20181221_WB_B_19011519_767.jpg

GTX101235 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with E2F1 antibody [N1N3] (GTX101235) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101235_WB_19011506_801.jpg

GTX101235 WB Image

Sample (30 µg of whole cell lysate)
A: A431 (GTX27909)
10% SDS PAGE
GTX101235 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101235_39812_IP_19011506_492.jpg

GTX101235 IP Image

E2F1 antibody [N1N3] immunoprecipitates E2F1 protein in IP experiments.
IP samples: HeLa whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of E2F1 protein by 4 μg E2F1 antibody [N1N3] (GTX101235)
10 % SDS-PAGE
The immunoprecipitated E2F1 protein was detected by E2F1 antibody [N1N3] (GTX101235) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

GTX101235_39812_20151216_IFA_19011506_570.jpg

GTX101235 ICC/IF Image

E2F1 antibody [N1N3] detects E2F1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: E2F1 protein stained by E2F1 antibody [N1N3] (GTX101235) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

GTX101235_39812_20151231_WB_M_19011506_160.jpg

GTX101235 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with E2F1 antibody (GTX101235) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf