GeneTex
United States (US)

E2F1 antibody

E2F1-antibody_WB_GTX14770-1_18121410_222.jpg

Cat No. GTX14770

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP, IHC
Reactivity Human
APPLICATION

Application Note

IHC: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at a dilution of 1/1000. Detects a band of approximately 53 kDa. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

47 kDa. ( Note )

Specificity/Sensitivity

The antibody does not cross reacts with other proteins of the E2F family members.
PROPERTIES

Form

Liquid

Buffer

Preservative: 0.02% Thimerosal / MerthiolateConstituents: PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide: SGLLPEEFISLSPPHEALDYHFG, corresponding to amino acids 392-414 of Human E2F1.

Purification

Immunogen affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

E2F Transcription Factor 1,E2F-1,Rbap1,Rbbp3,Rbp3,E2F1

Cellular Localization

Nuclear

Background

E2F's are DNA binding proteins, which associate with negative regulators, such as the retinoblastoma p107 protein, resulting in an altered rate of gene transcription. E2F1 is proposed to be involved in several cellular processes that range from tumor suppressor, cell progression and oncogenesis. E2F1 overexpression can also drive cells into apoptosis.

Database

Research Area

DATA IMAGES
E2F1-antibody_WB_GTX14770-1_18121410_222.jpg

GTX14770 WB Image

Western blot of E2F1 antibody with GTX14770 sample. Antibody dilution 1:500 in diluObuffer