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E2F3 antibody [PG30]

Orthogonal
Anti-E2F3 antibody [PG30] used in Western Blot (WB). GTX11843
Anti-E2F3 antibody [PG30] used in Western Blot (WB). GTX11843

Cat. No. GTX11843

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 PG30

Isotype

 IgG2a

Application

 WB, IP

Reactivity

 Human
Package
100 μl ($429)
See all E2F3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 4-8 μg/ml
IP Assay dependent
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

~2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

recombinant human E2F3.

Purification

Purified immunoglobulin

Conjugation

Unconjugated

RRID

AB_370173

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

E2F transcription factor 3 , E2F-3

Cellular Localization

Nucleus

Background

E2F transcription factors are functionally regulated by binding to Rb p110, p107, and p130. E2F 3 is regulated by complex formation with Rb p110. E2F family members bind DNA as heterodimers with members of the DP family of polypeptides.

Database

Research Area

DATA IMAGES

Anti-E2F3 antibody [PG30] used in Western Blot (WB). GTX11843

GTX11843 WB Image

WB analysis of 293T cell lysates using GTX11843 E2F3 antibody [PG30].
Dilution :
Lane1 : 4 μg/mL
Lane2 : 8 μg/mL

Anti-E2F3 antibody [PG30] used in Western Blot (WB). GTX11843

GTX11843 WB Image

Orthogonal

The data was published in the journal NPJ Breast Cancer in 2020. PMID: 31934613

REFERENCE

Application Reference

Daniele Simoneschi et al. Nature 2021; 592 (7856) : 789-793 CRL4AMBRA1 is a master regulator of D-type cyclins.
Application : WB
Reactivity : Human
Giro-Perafita A et al. NPJ Breast Cancer 2020; 6 : 1 LncRNA RP11-19E11 is an E2F1 target required for proliferation and survival of basal breast cancer.
Clijsters L et al. Mol Cell 2019; 74 (6) : 1264-1277.e7 Cyclin F Controls Cell-Cycle Transcriptional Outputs by Directing the Degradation of the Three Activator E2Fs.
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 429