Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1 - 2 μg/mL |
5 μg/mL |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Human Lymphoid Tissue Tissue Slide(GTX21833) , Human Lymph node Tissue Lysate(GTX29055)
Product Note
At least three isoforms of E2F3 are known to exist; this antibody will detect only the largest isoform. This antibody is predicted to not cross-react with other members of the E2F transcription factor family.
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
E2F3 antibody was raised against a 15 amino acid peptide near the center of human E2F3.The immunogen is located within amino acids 150 - 200 of E2F3.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
E2F transcription factor 3 , E2F-3
Cellular Localization
Nucleus
Background
This gene encodes a member of a small family of transcription factors that function through binding of DP interaction partner proteins. The encoded protein recognizes a specific sequence motif in DNA and interacts directly with the retinoblastoma protein (pRB) to regulate the expression of genes involved in the cell cycle. Altered copy number and activity of this gene have been observed in a number of human cancers. There are pseudogenes for this gene on chromosomes 2 and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]
Database
Research Area