GeneTex
United States (US)

E2F3 antibody

GTX31589_2774_WB_20180221_18121410_832.jpg
GTX31589_1230_IHC-P_20180221_18121410_891.jpg
GTX31589_1229_IHC-P_20180221_18121410_785.jpg

Cat No. GTX31589

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

Human Lymphoid Tissue Tissue Slide(GTX21833) , Human Lymph node Tissue Lysate(GTX29055)

Specificity/Sensitivity

At least three isoforms of E2F3 are known to exist; this antibody will detect only the largest isoform. This antibody is predicted to not cross-react with other members of the E2F transcription factor family.
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

E2F3 antibody was raised against a 15 amino acid peptide near the center of human E2F3.The immunogen is located within amino acids 150 - 200 of E2F3.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

E2F Transcription Factor 3,E2F-3,E2F3

Background

This gene encodes a member of a small family of transcription factors that function through binding of DP interaction partner proteins. The encoded protein recognizes a specific sequence motif in DNA and interacts directly with the retinoblastoma protein (pRB) to regulate the expression of genes involved in the cell cycle. Altered copy number and activity of this gene have been observed in a number of human cancers. There are pseudogenes for this gene on chromosomes 2 and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]

Database

Research Area

DATA IMAGES
GTX31589_2774_WB_20180221_18121410_832.jpg

GTX31589 WB Image

WB analysis of human lymph node tissue lysate using GTX31589 E2F3 antibody.
Working concentration : 1 μg/ml

GTX31589_1230_IHC-P_20180221_18121410_891.jpg

GTX31589 IHC-P Image

IHC-P analysis of human lymph node tissue using GTX31589 E2F3 antibody.
Working concentration : 20 μg/ml

GTX31589_1229_IHC-P_20180221_18121410_785.jpg

GTX31589 IHC-P Image

IHC-P analysis of human lymph node tissue using GTX31589 E2F3 antibody.
Working concentration : 5 μg/ml

Package List Price ($)
$ 329