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E2F4 antibody, C-term

Anti-E2F4 antibody, C-term used in Western Blot (WB). GTX77806
Anti-E2F4 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX77806
Anti-E2F4 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX77806

Cat. No. GTX77806

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
50 μg ($399)
See all E2F4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.2-2.5 ug/ml
IHC-P 2-10 ug/ml
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

HepG2

PROPERTIES

Form

Liquid

Buffer

PBS, 2% Sucrose

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a C-terminal region of Human E2F4

Purification

Affinity Purified

Conjugation

Unconjugated

RRID

AB_424754

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

E2F transcription factor 4 , E2F-4

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. It plays an important role in the suppression of proliferation-associated genes, and its gene mutation and increased expression may be associated with human cancer. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-E2F4 antibody, C-term used in Western Blot (WB). GTX77806

GTX77806 WB Image

WB analysis of HepG2 cells using GTX77806 E2F4 antibody at 0.2-1μg/ml.

Anti-E2F4 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX77806

GTX77806 IHC-P Image

IHC-P analysis of human intestine tissue using GTX77806 E2F4 antibody at 4.0-8.0μg/ml.

Anti-E2F4 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX77806

GTX77806 IHC-P Image

IHC-P analysis of human stomach tissue using GTX77806 E2F4 antibody at 4.0-8.0μg/ml.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399