E2F5 antibody, N-term

Cat. No. GTX80623

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P, FACS
Reactivity	Human
Package	400 μl ($399)

See all E2F5 products

Datasheet
Document

View product citations for antibody GTX80623 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:2000
IHC-P	1:50-1:100
FACS	1:10-1:50

Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Xenopus(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 66-93 amino acids from the N-terminal region of human E2F5.

Purification

Protein A purified, followed by peptide affinity purification.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

E2F transcription factor 5 , E2F-5

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionarily conserved domains that are present in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein is differentially phosphorylated and is expressed in a wide variety of human tissues. It has higher identity to E2F4 than to other family members. Both this protein and E2F4 interact with tumor suppressor proteins p130 and p107, but not with pRB. Alternative splicing results in multiple variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Database

Gene ID: 1875 E2F5
UniProt: Q15329 E2F5

Research Area

DATA IMAGES

GTX80623 WB Image

WB analysis of HepG2 whole cell lysate using GTX80623 E2F5 antibody, N-term.
Loading : 20 μg per lane
Dilution : 1:2000

GTX80623 FACS Image

FACS analysis of ZR-75-1 cells using GTX80623 E2F5 antibody, N-term.
Top histogram : negative control
Bottom histogram : ZR-75-1 cells

GTX80623 IHC-P Image

IHC-P analysis of human hepatocarcinoma using GTX80623 E2F5 antibody, N-term.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf

Package

List Price ($)

400 μl

$ 399

Qty