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E2F5 antibody

KO/KD Orthogonal Overexpression
Anti-E2F5 antibody used in Western Blot (WB). GTX129491
Anti-E2F5 antibody used in Western Blot (WB). GTX129491
Anti-E2F5 antibody used in Western Blot (WB). GTX129491
Anti-E2F5 antibody used in Western Blot (WB). GTX129491

Cat. No. GTX129491

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all E2F5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

HA-human E2F5-transfected 293T cells , A549 , H1299 , HCT116 , mouse placenta , mouse leukocyte treated with CFA

Product Note

Knockdown/Knockout validation was supported by references (PMID:36883841)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human E2F5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886016

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

E2F transcription factor 5 , E2F-5

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionarily conserved domains that are present in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein is differentially phosphorylated and is expressed in a wide variety of human tissues. It has higher identity to E2F4 than to other family members. Both this protein and E2F4 interact with tumor suppressor proteins p130 and p107, but not with pRB. Alternative splicing results in multiple variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-E2F5 antibody used in Western Blot (WB). GTX129491

GTX129491 WB Image

Orthogonal

Untreated (–) and treated (+) Mouse leukocyte extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with E2F5 antibody (GTX129491) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-E2F5 antibody used in Western Blot (WB). GTX129491

GTX129491 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with E2F5 antibody (GTX129491) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-E2F5 antibody used in Western Blot (WB). GTX129491

GTX129491 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with E2F5 antibody (GTX129491) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-E2F5 antibody used in Western Blot (WB). GTX129491

GTX129491 WB Image

Overexpression

E2F5 antibody detects E2F5 protein by western blot analysis.
A. 30 μg 293T whole cell extract
B. 30 μg whole cell extract of HA-human E2F5-transfected 293T cells
10 % SDS-PAGE
E2F5 antibody (GTX129491) dilution: 1:10000

REFERENCE

Application Reference

Taverniti V et al. mSphere 2023; 8 (2) : e0005623 The E2F4/p130 Repressor Complex Cooperates with Oncogenic ΔNp73α To Inhibit Gene Expression in Human Papillomavirus 38 E6/E7-Transformed Keratinocytes and in Cancer Cells.
Application : WB
Inagaki Y et al. Oncol Rep 2020; 44 (5) : 2241-2252 Knockdown of E2F5 induces cell death via the TP53?dependent pathway in breast cancer cells carrying wild?type TP53.
Application : WB
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169