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E2F6 antibody

Anti-E2F6 antibody used in IHC (Paraffin sections) (IHC-P). GTX114373
Anti-E2F6 antibody used in Western Blot (WB). GTX114373
Anti-E2F6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114373

Cat. No. GTX114373

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all E2F6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

A549

Predict Reactivity

Mouse, Rat, Bovine, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human E2F6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619933

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

E2F transcription factor 6 , E2F-6

Cellular Localization

Nucleus

Background

This gene encodes a member of the E2F transcription factor protein family. E2F family members play a crucial role in control of the cell cycle and of the action of tumor suppressor proteins. They are also a target of the transforming proteins of small DNA tumor viruses. Many E2F proteins contain several evolutionarily conserved domains: a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. The encoded protein of this gene is atypical because it lacks the transactivation and tumor suppressor protein association domains. It contains a modular suppression domain and is an inhibitor of E2F-dependent transcription. The protein is part of a multimeric protein complex that contains a histone methyltransferase and the transcription factors Mga and Max. Multiple transcript variants have been reported for this gene, but it has not been clearly demonstrated that they encode valid isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-E2F6 antibody used in IHC (Paraffin sections) (IHC-P). GTX114373

GTX114373 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H441 xenograft, using E2F6(GTX114373) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-E2F6 antibody used in Western Blot (WB). GTX114373

GTX114373 WB Image

Sample (30 ug of whole cell lysate)
A: A549
12% SDS PAGE
E2F6 antibody
GTX114373 diluted at 1:1000

Anti-E2F6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114373

GTX114373 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A549, using E2F6(GTX114373) antibody at 1:500 dilution.

REFERENCE

Application Reference

Kai Huang et al. Advanced Science 2019; (Epub) Genome?Wide CRISPR?Cas9 Screening Identifies NF??eB/E2F6 Responsible for EGFRvIII?Associated Temozolomide Resistance in Glioblastoma.
Application : WB, ICC/IF, IHC-P
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169