GeneTex
  • Country / Location Selection

United States (US)

E2F6 antibody

Anti-E2F6 antibody used in IHC (Paraffin sections) (IHC-P). GTX33167
Anti-E2F6 antibody used in Immunoprecipitation (IP). GTX33167
Anti-E2F6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33167
Anti-E2F6 antibody used in ChIP assay (ChIP assay). GTX33167
Anti-E2F6 antibody used in Western Blot (WB). GTX33167

Cat No. GTX33167

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP, ChIP assay

Reactivity

Human, Mouse
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:20-1:100
IHC-P 1:50-1:200
IP 1:50-1:100
ChIP assay 1:20-1:50
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-281 of human E2F6 (NP_937987.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

E2F transcription factor 6 , E2F-6

Cellular Localization

Nucleus

Background

This gene encodes a member of a family of transcription factors that play a crucial role in the control of the cell cycle. The protein encoded by this gene lacks the transactivation and tumor suppressor protein association domains found in other family members, and contains a modular suppression domain that functions in the inhibition of transcription. It interacts in a complex with chromatin modifying factors. There are pseudogenes for this gene on chromosomes 22 and X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]

Database

Research Area

DATA IMAGES

Anti-E2F6 antibody used in IHC (Paraffin sections) (IHC-P). GTX33167

GTX33167 IHC-P Image

IHC-P analysis of mouse lung tissue using GTX33167 E2F6 antibody.
Dilution : 1:200

Anti-E2F6 antibody used in Immunoprecipitation (IP). GTX33167

GTX33167 IP Image

IP analysis of MCF7 cells using GTX33167 E2F6 antibody.
IP reaction : 200μg extracts / 3μg antibody antibody
Dilution : 1:500

Anti-E2F6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33167

GTX33167 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX33167 E2F6 antibody.
Blue : DAPI

Anti-E2F6 antibody used in ChIP assay (ChIP assay). GTX33167

GTX33167 ChIP assay Image

ChIP analysis of HeLa cells using GTX33167 E2F6 antibody. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-E2F6 antibody used in Western Blot (WB). GTX33167

GTX33167 WB Image

WB analysis of various sample lysates using GTX33167 E2F6 antibody.
Loading : 25ug per lane
Dilution : 1:1000

REFERENCE

There are currently no references for E2F6 antibody (GTX33167). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for E2F6 antibody (GTX33167). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf