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United States (US)

E2F6 antibody

Anti-E2F6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33167
Anti-E2F6 antibody used in Immunoprecipitation (IP). GTX33167
Anti-E2F6 antibody used in Western Blot (WB). GTX33167
Anti-E2F6 antibody used in IHC (Paraffin sections) (IHC-P). GTX33167

Cat No. GTX33167

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:20 - 1:100
IHC-P 1:50 - 1:200
IP 1:20 - 1:100
Not tested in other applications.

Calculated MW

32 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-281 of human E2F6 (NP_937987.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

E2F Transcription Factor 6,E2F-6,E2F6

Background

This gene encodes a member of a family of transcription factors that play a crucial role in the control of the cell cycle. The protein encoded by this gene lacks the transactivation and tumor suppressor protein association domains found in other family members, and contains a modular suppression domain that functions in the inhibition of transcription. It interacts in a complex with chromatin modifying factors. There are pseudogenes for this gene on chromosomes 22 and X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]

Database

Research Area

DATA IMAGES
Anti-E2F6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33167

GTX33167 ICC/IF Image

ICC/IF analysis of HeLa cell using GTX33167 E2F6 antibody.

Anti-E2F6 antibody used in Immunoprecipitation (IP). GTX33167

GTX33167 IP Image

IP analysis of MCF-7 cell lysate using GTX33167 E2F6 antibody.
IP reaction : 3μg antibody / 200μg cell lysate
Dilution : 1:500

Anti-E2F6 antibody used in Western Blot (WB). GTX33167

GTX33167 WB Image

WB analysis of various samples using GTX33167 E2F6 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-E2F6 antibody used in IHC (Paraffin sections) (IHC-P). GTX33167

GTX33167 IHC-P Image

IHC-P analysis of mouse lung tissue using GTX33167 E2F6 antibody.
Dilution : 1:200

Package List Price ($)
$ 329