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EAAT1 antibody

Anti-EAAT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX131623
Anti-EAAT1 antibody used in Western Blot (WB). GTX131623
Anti-EAAT1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX131623

Cat. No. GTX131623

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr

Reactivity

 Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all EAAT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

rat brain

Predict Reactivity

Human, Bovine, Dog, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Intracellular domain of human EAAT1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886506

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

solute carrier family 1 member 3 , EA6 , EAAT1 , GLAST , GLAST1

Cellular Localization

Cell membrane

Background

This gene encodes a member of a member of a high affinity glutamate transporter family. This gene functions in the termination of excitatory neurotransmission in central nervous system. Mutations are associated with episodic ataxia, Type 6. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Feb 2014]

Database

Research Area

DATA IMAGES

Anti-EAAT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX131623

GTX131623 IHC-P Image

EAAT1 antibody detects EAAT1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: EAAT1 stained by EAAT1 antibody (GTX131623) diluted at 1:750.
Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-EAAT1 antibody used in Western Blot (WB). GTX131623

GTX131623 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with EAAT1 antibody (GTX131623) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EAAT1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX131623

GTX131623 IHC-Fr Image

EAAT1 antibody detects EAAT1 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse mouse cerebellum.
Green: EAAT1 stained by EAAT1 antibody (GTX131623) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169