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EAAT1 antibody

Anti-EAAT1 antibody used in Western Blot (WB). GTX134059
Anti-EAAT1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX134059
Anti-EAAT1 antibody used in Western Blot (WB). GTX134059
Anti-EAAT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX134059

Cat No. GTX134059

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, IHC-Fr

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

mouse cerebellum , rat cerebellum

Predict Reactivity

Bovine, Dog, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.15 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of mouse EAAT1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887194

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

solute carrier family 1 (glial high affinity glutamate transporter), member 3 , AI504299 , B430115D02Rik , Eaat1 , GLAST , GLAST-1 , GLU-T , GluT-1 , Gmt1 , MGluT1

Cellular Localization

Membrane

Background

Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.

Database

Research Area

DATA IMAGES

Anti-EAAT1 antibody used in Western Blot (WB). GTX134059

GTX134059 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with EAAT1 antibody (GTX134059) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-EAAT1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX134059

GTX134059 IHC-Fr Image

EAAT1 antibody detects EAAT1 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse cerebellum.
Green: EAAT1 stained by EAAT1 antibody (GTX134059) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-EAAT1 antibody used in Western Blot (WB). GTX134059

GTX134059 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with EAAT1 antibody (GTX134059) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EAAT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX134059

GTX134059 IHC-P Image

EAAT1 antibody detects EAAT1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: EAAT1 stained by EAAT1 antibody (GTX134059) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, a cytoplasm marker, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169