EAAT1 antibody

EAAT1 antibody detects EAAT1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: EAAT1 stained by EAAT1 antibody (GTX134060) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, a cytoplasm marker, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

EAAT1 antibody detects EAAT1 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse cerebellum.
Green: EAAT1 stained by EAAT1 antibody (GTX134060) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

EAAT1 antibody detects EAAT1 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EAAT1 stained by EAAT1 antibody (GTX134060) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with EAAT1 antibody (GTX134060) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.