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EAAT1 antibody

Overexpression
Anti-EAAT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX20262
Anti-EAAT1 antibody used in Western Blot (WB). GTX20262
Anti-EAAT1 antibody used in Flow cytometry (FACS). GTX20262
Anti-EAAT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX20262

Cat. No. GTX20262

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, ELISA

Reactivity

 Human, Mouse, Rat, Bovine
Package
100 μl ($409)
See all EAAT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 2 μg/ml
ICC/IF 1:10 - 1:500
IHC-P 1:50 - 1:500
IHC-Fr 1:10 - 1:500
FACS 1:200 - 1:500
ELISA 1:100 - 1:2000
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

A synthetic peptide made to a C-terminal portion of the rat SLC1A3 protein (between residues 500-542) [UniProt P24942]

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887574

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

solute carrier family 1 member 3 , EAAT1 , GLAST , GLAST-1 , GluT-1

Cellular Localization

Cell membrane

Background

carries out Na+ dependent transport of glutamate and aspartate; may regulate neurotransmitter concentration in the central nervous system [RGD]

Database

Research Area

DATA IMAGES

Anti-EAAT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX20262

GTX20262 ICC/IF Image

ICC/IF analysis of Neuro2a cells using GTX20262 EAAT1 antibody.
Green : primary antibody
Red : Tubulin
Blue : DAPI
Dilution : 1:250

Anti-EAAT1 antibody used in Western Blot (WB). GTX20262

GTX20262 WB Image

WB analysis of (1) human, (2) mouse, and (3) rat brain tissue lysate using GTX20262 EAAT1 antibody.

Anti-EAAT1 antibody used in Flow cytometry (FACS). GTX20262

GTX20262 FACS Image

FACS (Intracellular staining) analysis of HEK293 cells using GTX20262 EAAT1 antibody.
Orange : Primary antibody
Blue : isotype control
Dilution : 1:500

Anti-EAAT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX20262

GTX20262 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX20262 EAAT1 antibody.

REFERENCE

Application Reference

Hart ML et al. Elife 2023; 12 Mitochondrial redox adaptations enable alternative aspartate synthesis in SDH-deficient cells.
Application : WB
Reactivity : Human
Li RQ et al. Neural Regen Res 2018; 13 (6) : 1013-1018 Catgut implantation at acupoints increases the expression of glutamate aspartate transporter and glial glutamate transporter-1 in the brain of rats with spasticity after stroke.
Garcia-Bermudez J et al. Nat Cell Biol 2018; 20 (7) : 775-781 Aspartate is a limiting * for cancer cell proliferation under hypoxia and in tumours.
Application : WB, ICC/IF
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409