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EAAT2 antibody

Anti-EAAT2 antibody used in IHC (Paraffin sections) (IHC-P). GTX134062
Anti-EAAT2 antibody used in Western Blot (WB). GTX134062
Anti-EAAT2 antibody used in IHC (Paraffin sections) (IHC-P). GTX134062

Cat No. GTX134062

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Human, Cat, Dog, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.77 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of mouse EAAT2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887196

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

solute carrier family 1 (glial high affinity glutamate transporter), member 2 , 1700091C19Rik , 2900019G14Rik , AI159670 , Eaat2 , GLT-1 , GLT1 , MGLT1

Cellular Localization

Membrane

Background

Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.

Database

Research Area

DATA IMAGES

Anti-EAAT2 antibody used in IHC (Paraffin sections) (IHC-P). GTX134062

GTX134062 IHC-P Image

EAAT2 antibody detects EAAT2 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: EAAT2 stained by EAAT2 antibody (GTX134062) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, a cytoplasm marker, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-EAAT2 antibody used in Western Blot (WB). GTX134062

GTX134062 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EAAT2 antibody (GTX134062) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EAAT2 antibody used in IHC (Paraffin sections) (IHC-P). GTX134062

GTX134062 IHC-P Image

EAAT2 antibody detects EAAT2 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hippocampus.
EAAT2 stained by EAAT2 antibody (GTX134062) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

There are currently no references for EAAT2 antibody (GTX134062). Be the first to share your publications with this product.

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169