GeneTex
United States (US)

EB1 antibody [N1C3]

GTX106259_WB_19011506_578.jpg

Cat No. GTX106259

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Positive Control

293T , A431 , Jurkat , Raji

Predict Reactivity

Mouse, Rat, Bovine, Dog, Chicken, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.87mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EB1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

EB1 antibody, MGC117374 antibody, MGC129946 antibody, MAPRE1 antibody, microtubule-associated protein RP/EB family member 1 antibody, APC-binding protein EB1 antibody, end-binding protein 1 antibody, adenomatous polyposis coli-binding protein EB1 antibody, "microtubule-associated protein, RP/EB family, member 1 antibody"

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene was first identified by its binding to the APC protein which is often mutated in familial and sporadic forms of colorectal cancer. This protein localizes to microtubules, especially the growing ends, in interphase cells. During mitosis, the protein is associated with the centrosomes and spindle microtubules. The protein also associates with components of the dynactin complex and the intermediate chain of cytoplasmic dynein. Because of these associations, it is thought that this protein is involved in the regulation of microtubule structures and chromosome stability. This gene is a member of the RP/EB family. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX106259_WB_19011506_578.jpg

GTX106259 WB Image

Sample (30 ug of whole cell lysate)

A: JurKat

12% SDS PAGE

GTX106259 diluted at 1:500

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169