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EB1 antibody

Anti-EB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX03450
Anti-EB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03450
Anti-EB1 antibody used in Western Blot (WB). GTX03450
Anti-EB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX03450

Cat. No. GTX03450

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399)
See all EB1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF 1:100-1:500
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Predict Reactivity

Rabbit, Bovine, Dog, Pig, Horse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human MAPRE1(Accession Q15691), corresponding to amino acid residues G176-N201.

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

microtubule associated protein RP/EB family member 1 , EB1

Cellular Localization

Cytoplasm, cytoskeleton , Golgi apparatus

Background

The protein encoded by this gene was first identified by its binding to the APC protein which is often mutated in familial and sporadic forms of colorectal cancer. This protein localizes to microtubules, especially the growing ends, in interphase cells. During mitosis, the protein is associated with the centrosomes and spindle microtubules. The protein also associates with components of the dynactin complex and the intermediate chain of cytoplasmic dynein. Because of these associations, it is thought that this protein is involved in the regulation of microtubule structures and chromosome stability. This gene is a member of the RP/EB family. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-EB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX03450

GTX03450 IHC-P Image

IHC-P analysis of rat colorectal tissue using GTX03450 EB1 antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

Anti-EB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03450

GTX03450 ICC/IF Image

ICC/IF analysis of PFA-fixed HeLa cells using GTX03450 EB1 antibody.
Red : Primary antibody
Green : beta tubulin
Blue : DAPI
Permeabilization : 0.1% Triton X-100
Dilution : 1:200

Anti-EB1 antibody used in Western Blot (WB). GTX03450

GTX03450 WB Image

WB analysis of rat brain tissue lysate using GTX03450 EB1 antibody. The lane on the left was treated with blocking peptide.

Anti-EB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX03450

GTX03450 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX03450 EB1 antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

REFERENCE

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REVIEW

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Package List Price ($)
$ 399