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EBP1 antibody [N3C3]

Anti-EBP1 antibody [N3C3] used in Western Blot (WB). GTX114610
Anti-EBP1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX114610
Anti-EBP1 antibody [N3C3] used in Western Blot (WB). GTX114610
Anti-EBP1 antibody [N3C3] used in Western Blot (WB). GTX114610
Anti-EBP1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114610

Cat. No. GTX114610

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Zebrafish
Package
100 μl ($419),
25 μl ($169)
See all EBP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

Mouse brain , A431

Predict Reactivity

Rat, Bovine, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.55 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EBP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619607

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

proliferation-associated 2G4 , EBP1 , HG4-1 , p38-2G4

Cellular Localization

Cytoplasm , Nucleus , nucleolus

Background

This gene encodes an RNA-binding protein that is involved in growth regulation. This protein is present in pre-ribosomal ribonucleoprotein complexes and may be involved in ribosome assembly and the regulation of intermediate and late steps of rRNA processing. This protein can interact with the cytoplasmic domain of the ErbB3 receptor and may contribute to transducing growth regulatory signals. This protein is also a transcriptional co-repressor of androgen receptor-regulated genes and other cell cycle regulatory genes through its interactions with histone deacetylases. This protein has been implicated in growth inhibition and the induction of differentiation of human cancer cells. Six pseudogenes, located on chromosomes 3, 6, 9, 18, 20 and X, have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-EBP1 antibody [N3C3] used in Western Blot (WB). GTX114610

GTX114610 WB Image

Sample (30 μg of whole cell lysate)
A: zebrafish eye
10% SDS PAGE
GTX114610 diluted at 1:1000

Anti-EBP1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX114610

GTX114610 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using EBP1 antibody [N3C3] (GTX114610) at 1:300 dilution.

Anti-EBP1 antibody [N3C3] used in Western Blot (WB). GTX114610

GTX114610 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX114610 diluted at 1:1000

Anti-EBP1 antibody [N3C3] used in Western Blot (WB). GTX114610

GTX114610 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
10% SDS PAGE
GTX114610 diluted at 1:1000

Anti-EBP1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114610

GTX114610 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using EBP1(GTX114610) antibody at 1:200 dilution.

REFERENCE

Application Reference

Wu JY et al. Cancers (Basel) 2019; 11 (5) YC-1 Antagonizes Wnt/帣-Catenin Signaling Through the EBP1 p42 Isoform in Hepatocellular Carcinoma.
Application : ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 419
$ 169