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EBP1 antibody

Anti-EBP1 antibody used in Immunoprecipitation (IP). GTX103791
Anti-EBP1 antibody used in Western Blot (WB). GTX103791

Cat. No. GTX103791

Reference 1
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human, Xenopus laevis
Package
100 μl ($399),
25 μl ($169)
See all EBP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF Assay dependent
IP 1:100-1:500
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

A431 , Jurkat

Predict Reactivity

Mouse, Rat, Bovine, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EBP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11165803

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

proliferation-associated 2G4 , EBP1 , HG4-1 , p38-2G4

Cellular Localization

Cytoplasm , Nucleus , nucleolus

Background

This gene encodes an RNA-binding protein that is involved in growth regulation. This protein is present in pre-ribosomal ribonucleoprotein complexes and may be involved in ribosome assembly and the regulation of intermediate and late steps of rRNA processing. This protein can interact with the cytoplasmic domain of the ErbB3 receptor and may contribute to transducing growth regulatory signals. This protein is also a transcriptional co-repressor of androgen receptor-regulated genes and other cell cycle regulatory genes through its interactions with histone deacetylases. This protein has been implicated in growth inhibition and the induction of differentiation of human cancer cells. Six pseudogenes, located on chromosomes 3, 6, 9, 18, 20 and X, have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-EBP1 antibody used in Immunoprecipitation (IP). GTX103791

GTX103791 IP Image

EBP1 antibody immunoprecipitates EBP1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of EBP1 protein by 2 μg of EBP1 antibody (GTX103791) 7.5% SDS-PAGE The immunoprecipitated EBP1 protein was detected by EBP1 antibody (GTX103791) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-EBP1 antibody used in Western Blot (WB). GTX103791

GTX103791 WB Image

Sample (30 ug of whole cell lysate)
A: A431
B: Jurkat
10% SDS PAGE
GTX103791 diluted at 1:10000

REFERENCE

Application Reference

Xu YM et al. Toxicol Res (Camb) 2019; 8 (6) : 994-1001 Cadmium telluride quantum dot-exposed human bronchial epithelial cells: a further study of the cellular response by proteomics.
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REVIEW

EBP1 antibody Cat. No. GTX103791

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Anti-EBP1 antibody used in Western Blot (WB). GTX103791
Date : Anonymous submitted on 07-Oct-2022
Score :
Application Tested : WB
Sample Species : X. laevis
Sample : Zenopus Egg Extract
Amount used : 10μg
Blocking : BSA, 26°C, 0.1Hr
Primary Antibody : 1:1000, 26°C, 0.5Hr
Anti-EBP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103791
Date : Anonymous submitted on 07-Oct-2022
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : HeLa cells
Blocking : BSA, 26°C, 0.1Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : Tween-20
Primary Antibody : 1:1000, 26°C, 0.5Hr
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169