APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , HepG2 , 293T
Predict Reactivity
Rat, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human ECH1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2036818
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
enoyl-CoA hydratase 1 , HPXEL
Cellular Localization
Mitochondrion , Peroxisome
Background
This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-ECH1 antibody [N1C3] used in Western Blot (WB). GTX103552](/templates/default/js/lightbox/images/loading.gif "Anti-ECH1 antibody [N1C3] used in Western Blot (WB). GTX103552")
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GTX103552 WB Image
Wild-type (WT) and ECH1 knockout (KO) 293T cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ECH1 antibody (GTX103552) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX103552 IHC-P Image
Immunohistochemical analysis of paraffin-embedded OV90 xenograft, using ECH1(GTX103552) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX103552 WB Image
Sample (30 ug of whole cell lysate)
A: 293T
B: Hep G2 (GTX27900)
10% SDS PAGE
GTX103552 diluted at 1:1000
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GTX103552 IHC-P Image
ECH1 antibody [N1C3] detects ECH1 protein at cytoplasm in mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
ECH1 antibody [N1C3] (GTX103552) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
There are currently no references for ECH1 antibody [N1C3] (GTX103552). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ECH1 antibody [N1C3] (GTX103552). Be the first to share your experience with this product.
![Anti-ECH1 antibody [N1C3] used in Western Blot (WB). GTX103552](/upload/website/prouct_img/small/GTX103552/GTX103552_40121_20171229_WB_KO_watermark_w_23060119_460.webp "Anti-ECH1 antibody [N1C3] used in Western Blot (WB). GTX103552")
![Anti-ECH1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX103552](/upload/website/prouct_img/small/GTX103552/GTX103552_IHC_w_23060119_181.webp "Anti-ECH1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX103552")
![Anti-ECH1 antibody [N1C3] used in Western Blot (WB). GTX103552](/upload/website/prouct_img/small/GTX103552/GTX103552_WB_w_23060119_299.webp "Anti-ECH1 antibody [N1C3] used in Western Blot (WB). GTX103552")
![Anti-ECH1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX103552](/upload/website/prouct_img/small/GTX103552/GTX103552_40121_20150529_IHC_M_w_23060119_240.webp "Anti-ECH1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX103552")
![Anti-ECH1 antibody [N1C3] used in Western Blot (WB). GTX103552](/upload/website/prouct_img/normal/GTX103552/GTX103552_40121_20171229_WB_KO_watermark_w_23060119_460.webp "Anti-ECH1 antibody [N1C3] used in Western Blot (WB). GTX103552")
![Anti-ECH1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX103552](/upload/website/prouct_img/normal/GTX103552/GTX103552_IHC_w_23060119_181.webp "Anti-ECH1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX103552")
![Anti-ECH1 antibody [N1C3] used in Western Blot (WB). GTX103552](/upload/website/prouct_img/normal/GTX103552/GTX103552_WB_w_23060119_299.webp "Anti-ECH1 antibody [N1C3] used in Western Blot (WB). GTX103552")
![Anti-ECH1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX103552](/upload/website/prouct_img/normal/GTX103552/GTX103552_40121_20150529_IHC_M_w_23060119_240.webp "Anti-ECH1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX103552")