GeneTex
United States (US)

ECH1 antibody [N1C3]

KO/KD
GTX103552_40121_20171229_WB_KO_watermark_19011506_448.jpg
GTX103552_WB_19011506_711.jpg
GTX103552_40121_20150529_IHC_M_19011506_693.jpg
GTX103552_IHC_19011506_547.jpg

Cat No. GTX103552

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
See all ECH1 products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji

Predict Reactivity

Rat, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ECH1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Enoyl-Coa Hydratase 1,Hpxel,Ech1

Cellular Localization

Mitochondrion , Peroxisome

Background

This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX103552_40121_20171229_WB_KO_watermark_19011506_448.jpg

GTX103552 WB Image

KO/KD

Wild-type (WT) and ECH1 knockout (KO) 293T cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with ECH1 antibody (GTX103552) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by www.edigene.com

GTX103552_WB_19011506_711.jpg

GTX103552 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: Hep G2 (GTX27900)
10% SDS PAGE
GTX103552 diluted at 1:1000

GTX103552_40121_20150529_IHC_M_19011506_693.jpg

GTX103552 IHC-P Image

ECH1 antibody [N1C3] detects ECH1 protein at cytoplasm in mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
ECH1 antibody [N1C3] (GTX103552) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX103552_IHC_19011506_547.jpg

GTX103552 IHC-P Image

Immunohistochemical analysis of paraffin-embedded OV90 xenograft, using ECH1(GTX103552) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169