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ECHS1 antibody [N1C2]

Anti-ECHS1 antibody [N1C2] used in Immunoprecipitation (IP). GTX114376
Anti-ECHS1 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114376
Anti-ECHS1 antibody [N1C2] used in Western Blot (WB). GTX114376
Anti-ECHS1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX114376
Anti-ECHS1 antibody [N1C2] used in Western Blot (WB). GTX114376
Anti-ECHS1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX114376
Anti-ECHS1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX114376
Anti-ECHS1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX114376
Anti-ECHS1 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114376
Anti-ECHS1 antibody [N1C2] used in Western Blot (WB). GTX114376

Cat. No. GTX114376

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all ECHS1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

Mouse brain , 293T , A431 , HeLa , HepG2

Predict Reactivity

Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.01 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ECHS1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10634171

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

enoyl-CoA hydratase, short chain 1 , ECHS1D , SCEH

Cellular Localization

Mitochondrion matrix

Background

The protein encoded by this gene functions in the second step of the mitochondrial fatty acid beta-oxidation pathway. It catalyzes the hydration of 2-trans-enoyl-coenzyme A (CoA) intermediates to L-3-hydroxyacyl-CoAs. The gene product is a member of the hydratase/isomerase superfamily. It localizes to the mitochondrial matrix. Transcript variants utilizing alternative transcription initiation sites have been described in the literature. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ECHS1 antibody [N1C2] used in Immunoprecipitation (IP). GTX114376

GTX114376 IP Image

Immunoprecipitation of ECHS1 protein from 293T whole cell extracts using 5 μg of ECHS1 antibody [N1C2] (GTX114376).
Western blot analysis was performed using ECHS1 antibody [N1C2] (GTX114376).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-ECHS1 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114376

GTX114376 ICC/IF Image

ECHS1 antibody [N1C2] detects ECHS1 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: ECHS1 protein stained by ECHS1 antibody [N1C2] (GTX114376) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-ECHS1 antibody [N1C2] used in Western Blot (WB). GTX114376

GTX114376 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX114376 diluted at 1:1000

Anti-ECHS1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX114376

GTX114376 IHC-P Image

ECHS1 antibody [N1C2] detects ECHS1 protein at mitochondria on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
ECHS1 antibody [N1C2] (GTX114376) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ECHS1 antibody [N1C2] used in Western Blot (WB). GTX114376

GTX114376 WB Image

ECHS1 antibody detects ECHS1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ECHS1 antibody (GTX114376) diluted by 1:5000.

Anti-ECHS1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX114376

GTX114376 IHC-P Image

ECHS1 antibody [N1C2] detects ECHS1 protein at mitochondria on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
ECHS1 antibody [N1C2] (GTX114376) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ECHS1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX114376

GTX114376 IHC-P Image

ECHS1 antibody [N1C2] detects ECHS1 protein at mitochondria on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
ECHS1 antibody [N1C2] (GTX114376) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ECHS1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX114376

GTX114376 IHC-P Image

ECHS1 antibody [N1C2] detects ECHS1 protein at mitochondria on mouse ovary by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
ECHS1 antibody [N1C2] (GTX114376) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ECHS1 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114376

GTX114376 ICC/IF Image

ECHS1 antibody [N1C2] detects ECHS1 protein at mitochondria by immunofluorescent analysis.
Sample: A431 cells were fixed in 2% paraformaldehyde/culture medium at RT for 30 min.
Green: ECHS1 protein stained by ECHS1 antibody [N1C2] (GTX114376) diluted at 1:200.
Blue: Hoechst 33342 staining.
Anti-ECHS1 antibody [N1C2] used in Western Blot (WB). GTX114376

GTX114376 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ECHS1 antibody [N1C2] (GTX114376) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Haack TB et al. Ann Clin Transl Neurol 2015; 2 (5) : 492-509 Deficiency of ECHS1 causes mitochondrial encephalopathy with cardiac involvement.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169