Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.01 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human ECHS1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10634171
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
enoyl-CoA hydratase, short chain 1 , ECHS1D , SCEH
Cellular Localization
Mitochondrion matrix
Background
The protein encoded by this gene functions in the second step of the mitochondrial fatty acid beta-oxidation pathway. It catalyzes the hydration of 2-trans-enoyl-coenzyme A (CoA) intermediates to L-3-hydroxyacyl-CoAs. The gene product is a member of the hydratase/isomerase superfamily. It localizes to the mitochondrial matrix. Transcript variants utilizing alternative transcription initiation sites have been described in the literature. [provided by RefSeq]
Immunoprecipitation of ECHS1 protein from 293T whole cell extracts using 5 μg of ECHS1 antibody [N1C2] (GTX114376). Western blot analysis was performed using ECHS1 antibody [N1C2] (GTX114376). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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GTX114376 ICC/IF Image
ECHS1 antibody [N1C2] detects ECHS1 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: ECHS1 protein stained by ECHS1 antibody [N1C2] (GTX114376) diluted at 1:500. Blue: Hoechst 33342 staining.
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GTX114376 WB Image
Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE GTX114376 diluted at 1:1000
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GTX114376 IHC-P Image
ECHS1 antibody [N1C2] detects ECHS1 protein at mitochondria on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. ECHS1 antibody [N1C2] (GTX114376) dilution: 1:500.
ECHS1 antibody detects ECHS1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ECHS1 antibody (GTX114376) diluted by 1:5000.
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GTX114376 IHC-P Image
ECHS1 antibody [N1C2] detects ECHS1 protein at mitochondria on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. ECHS1 antibody [N1C2] (GTX114376) dilution: 1:500.
ECHS1 antibody [N1C2] detects ECHS1 protein at mitochondria by immunofluorescent analysis. Sample: A431 cells were fixed in 2% paraformaldehyde/culture medium at RT for 30 min. Green: ECHS1 protein stained by ECHS1 antibody [N1C2] (GTX114376) diluted at 1:200. Blue: Hoechst 33342 staining.
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GTX114376 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ECHS1 antibody [N1C2] (GTX114376) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.