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ECHS1 antibody [N1C2]

Anti-ECHS1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX114376
Anti-ECHS1 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114376
Anti-ECHS1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX114376
Anti-ECHS1 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114376
Anti-ECHS1 antibody [N1C2] used in Immunoprecipitation (IP). GTX114376
Anti-ECHS1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX114376
Anti-ECHS1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX114376
Anti-ECHS1 antibody [N1C2] used in Western Blot (WB). GTX114376
Anti-ECHS1 antibody [N1C2] used in Western Blot (WB). GTX114376
Anti-ECHS1 antibody [N1C2] used in Western Blot (WB). GTX114376

Cat. No. GTX114376

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all ECHS1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

Mouse brain , 293T , A431 , HeLa , HepG2

Predict Reactivity

Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ECHS1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10634171

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

enoyl-CoA hydratase, short chain 1 , ECHS1D , SCEH

Cellular Localization

Mitochondrion matrix

Background

The protein encoded by this gene functions in the second step of the mitochondrial fatty acid beta-oxidation pathway. It catalyzes the hydration of 2-trans-enoyl-coenzyme A (CoA) intermediates to L-3-hydroxyacyl-CoAs. The gene product is a member of the hydratase/isomerase superfamily. It localizes to the mitochondrial matrix. Transcript variants utilizing alternative transcription initiation sites have been described in the literature. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ECHS1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX114376

GTX114376 IHC-P Image

ECHS1 antibody [N1C2] detects ECHS1 protein at mitochondria on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
ECHS1 antibody [N1C2] (GTX114376) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ECHS1 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114376

GTX114376 ICC/IF Image

ECHS1 antibody [N1C2] detects ECHS1 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: ECHS1 protein stained by ECHS1 antibody [N1C2] (GTX114376) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-ECHS1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX114376

GTX114376 IHC-P Image

ECHS1 antibody [N1C2] detects ECHS1 protein at mitochondria on mouse ovary by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
ECHS1 antibody [N1C2] (GTX114376) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ECHS1 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114376

GTX114376 ICC/IF Image

ECHS1 antibody [N1C2] detects ECHS1 protein at mitochondria by immunofluorescent analysis.
Sample: A431 cells were fixed in 2% paraformaldehyde/culture medium at RT for 30 min.
Green: ECHS1 protein stained by ECHS1 antibody [N1C2] (GTX114376) diluted at 1:200.
Blue: Hoechst 33342 staining.
Anti-ECHS1 antibody [N1C2] used in Immunoprecipitation (IP). GTX114376

GTX114376 IP Image

Immunoprecipitation of ECHS1 protein from 293T whole cell extracts using 5 μg of ECHS1 antibody [N1C2] (GTX114376).
Western blot analysis was performed using ECHS1 antibody [N1C2] (GTX114376).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-ECHS1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX114376

GTX114376 IHC-P Image

ECHS1 antibody [N1C2] detects ECHS1 protein at mitochondria on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
ECHS1 antibody [N1C2] (GTX114376) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ECHS1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX114376

GTX114376 IHC-P Image

ECHS1 antibody [N1C2] detects ECHS1 protein at mitochondria on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
ECHS1 antibody [N1C2] (GTX114376) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ECHS1 antibody [N1C2] used in Western Blot (WB). GTX114376

GTX114376 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ECHS1 antibody [N1C2] (GTX114376) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ECHS1 antibody [N1C2] used in Western Blot (WB). GTX114376

GTX114376 WB Image

ECHS1 antibody detects ECHS1 protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ECHS1 antibody (GTX114376) diluted by 1:5000.

Anti-ECHS1 antibody [N1C2] used in Western Blot (WB). GTX114376

GTX114376 WB Image

ECHS1 antibody detects ECHS1 protein by Western blot analysis. Mouse tissue extracts (50 μg) was separated by 12 % SDS-PAGE, and the membrane was blotted with ECHS1 antibody (GTX114376) diluted by 1:5000.

REFERENCE

Application Reference

Haack TB et al. Ann Clin Transl Neurol 2015; 2 (5) : 492-509 Deficiency of ECHS1 causes mitochondrial encephalopathy with cardiac involvement.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169