APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-2μg/ml |
| 0.5-1μg/ml for 30 minutes at RT |
| 0.5-1μg/10⁶ cells |
|
Note :
IHC-P
Staining of formalin-fixed tissues require boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
|
Not tested in other applications.
Calculated MW
Product Note
This MAb reacts with a reduction-resistant epitope present in both free and SIgA bound Secretory Component. It does not react with the cell lines lacking secretory component. The antibody is useful for studying the distribution and level of both free and bound secretory component.
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant full-length human ECM1 protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ECM1 , URBWD , extracellular matrix protein 1
Cellular Localization
Secreted,Extracellular space,Extracellular matrix
Background
This gene encodes a soluble protein that is involved in endochondral bone formation, angiogenesis, and tumor biology. It also interacts with a variety of extracellular and structural proteins, contributing to the maintenance of skin integrity and homeostasis. Mutations in this gene are associated with lipoid proteinosis disorder (also known as hyalinosis cutis et mucosae or Urbach-Wiethe disease) that is characterized by generalized thickening of skin, mucosae and certain viscera. Alternatively spliced transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq, Feb 2011]
Database
Research Area
DATA IMAGES
![Anti-ECM1 antibody [ECM1/792] used in IHC (Paraffin sections) (IHC-P). GTX34684](/templates/default/js/lightbox/images/loading.gif "Anti-ECM1 antibody [ECM1/792] used in IHC (Paraffin sections) (IHC-P). GTX34684")
|
GTX34684 IHC-P Image
IHC-P analysis of human colon carcinoma tissue using GTX34684 ECM1 antibody [ECM1/792].
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REFERENCE
There are currently no references for ECM1 antibody [ECM1/792] (GTX34684). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ECM1 antibody [ECM1/792] (GTX34684). Be the first to share your experience with this product.
![Anti-ECM1 antibody [ECM1/792] used in IHC (Paraffin sections) (IHC-P). GTX34684](/upload/website/prouct_img/small/GTX34684/GTX34684_20200115_IHC-P_474_w_23060801_591.webp "Anti-ECM1 antibody [ECM1/792] used in IHC (Paraffin sections) (IHC-P). GTX34684")
![Anti-ECM1 antibody [ECM1/792] used in IHC (Paraffin sections) (IHC-P). GTX34684](/upload/website/prouct_img/normal/GTX34684/GTX34684_20200115_IHC-P_474_w_23060801_591.webp "Anti-ECM1 antibody [ECM1/792] used in IHC (Paraffin sections) (IHC-P). GTX34684")