GeneTex
United States (US)

ECM1 antibody [ECM1/792]

GTX34684_20170712_IHC-P_18121410_984.jpg

Cat No. GTX34684

Host Mouse
Clonality Monoclonal
Clone Name ECM1/792
Isotype IgG1
Application IHC-P
Reactivity Human, Rat
APPLICATION

Application Note

IHC-P:0.5-1ug/ml for 30 minutes at RT.

Calculated MW

61 kDa. ( Note )

Specificity/Sensitivity

This MAb reacts with a reduction-resistant epitope present in both free and SIgA bound Secretory Component. It does not react with the cell lines lacking secretory component. The antibody is useful for studying the distribution and level of both free and bound secretory component. Secretory component is differentially expressed in epithelium, and the antibody is a popular marker for identifying subpopulations of epithelial cells and epithelial differentiation. The Secretory component antibody is a useful research tool for studying mucosal immunity, inflammation, remodeling, differentiation and tumorigenesis, all processes associated with differential secretory component expression.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant human ECM1 protein

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Extracellular Matrix Protein 1,Urbwd,Ecm1

Background

This gene encodes a soluble protein that is involved in endochondral bone formation, angiogenesis, and tumor biology. It also interacts with a variety of extracellular and structural proteins, contributing to the maintenance of skin integrity and homeostasis. Mutations in this gene are associated with lipoid proteinosis disorder (also known as hyalinosis cutis et mucosae or Urbach-Wiethe disease) that is characterized by generalized thickening of skin, mucosae and certain viscera. Alternatively spliced transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq, Feb 2011]

Database

Research Area

DATA IMAGES
GTX34684_20170712_IHC-P_18121410_984.jpg

GTX34684 IHC-P Image

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with IgA Secretory Component Monoclonal Antibody (ECM1/792).