ECSIT antibody

Cat. No. GTX85081

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P, ELISA
Reactivity	Human, Mouse
Package	100 μg ($399)

See all ECSIT products

Datasheet
Document

View product citations for antibody GTX85081 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	0.5 - 2 μg/mL
IHC-P	2 μg/mL
ELISA	Assay dependent

Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

Human Heart Tissue Lysate(GTX27916) , Mouse Heart Tissue Lysate(GTX27932)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

ECSIT antibody was raised against a peptide corresponding to 14 amino acids near the C-terminus of human ECSIT.The immunogen is located within amino acids 290 - 340 of ECSIT.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ECSIT signalling integrator , SITPEC

Cellular Localization

Cytoplasm,Nucleus,Mitochondrion

Background

Activation of NF-?B as a result of Toll-like receptor (TLR) and IL-1 receptor signaling is a major component of innate immune responses (reviewed in 1). Signals from these receptors are relayed by a number of adapter molecules such as TRIF, TIRAP, and MyD88 (2) to kinases such as IRAK (3) and other intermediates such as TNF receptor associated factor (TRAF)-6 (4). ECSIT (evolutionarily conserved signaling intermediate in Toll pathways) was initially identified as a cytoplasmic protein interacting specifically with TNF receptor associated factor (TRAF)-6 in the TLR pathway (5). Recently however, ECSIT has also been shown to be required for bone morphogenetic protein (Bmp) signaling and mesoderm formation during mouse embryogenesis (6), indicating the possibility of cross-talk between the TLR/IL-B and Bmp signaling pathways.

Database

Gene ID: 51295 ECSIT
UniProt: Q9BQ95 ECSIT

Research Area

DATA IMAGES

GTX85081 IHC-P Image

IHC-P analysis of mouse heart tissue using GTX85081 ECSIT antibody.
Working concentration : 2 μg/ml

GTX85081 WB Image

WB analysis of human heart tissue lysate using GTX85081 ECSIT antibody.
Working concentration : (A) 0.5, (B) 1, and (C) 2 μg/ml

REFERENCE

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SDS
PBS.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 399

Qty