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EDAR antibody [AT19E8]

Anti-EDAR antibody [AT19E8] used in Western Blot (WB). GTX57609
Anti-EDAR antibody [AT19E8] used in Flow cytometry (FACS). GTX57609

Cat. No. GTX57609

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AT19E8

Isotype

 IgG2a

Application

 WB, FACS

Reactivity

 Human
Package
100 μl ($399)
See all EDAR products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Recommended starting dilution is 1:1000.
FACS Assay dependent
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone AT19E8 is derived from hybridization of mouse F2 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human EDAR protein.

Purification

Protein A Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ectodysplasin A receptor , DL , ECTD10A , ECTD10B , ED1R , ED3 , ED5 , EDA1R , EDA3 , EDAA1R , EDAR , HRM1

Cellular Localization

Membrane

Background

This gene encodes a member of the tumor necrosis factor receptor family. The encoded transmembrane protein is a receptor for the soluble ligand ectodysplasin A, and can activate the nuclear factor-kappaB, JNK, and caspase-independent cell death pathways. It is required for the development of hair, teeth, and other ectodermal derivatives. Mutations in this gene result in autosomal dominant and recessive forms of hypohidrotic ectodermal dysplasia. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-EDAR antibody [AT19E8] used in Western Blot (WB). GTX57609

GTX57609 WB Image

WB analysis of various samples using GTX57609 EDAR antibody.
Lane 1 : Recombinant Human EDAR protein (50 ng)
Lane 2 : A549 whole cell lysate (40 μg)
Lane 3 : Ramos whole cell lysate (40 μg)

Anti-EDAR antibody [AT19E8] used in Flow cytometry (FACS). GTX57609

GTX57609 FACS Image

FACS analysis of HeLa cells using GTX57609 EDAR antibody.
Cell Number: 1 x 10⁶ cells
Red: Primary antibody
Antibody amount: 2-5 μg

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399