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EDAR antibody

Anti-EDAR antibody used in Western Blot (WB). GTX04637
Anti-EDAR antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04637
Anti-EDAR antibody used in IHC (Paraffin sections) (IHC-P). GTX04637

Cat. No. GTX04637

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all EDAR products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:3000
ICC/IF 1:100-1:500
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Predict Reactivity

Rabbit, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.4, 150 mM NaCl, 50% glycerol

Preservative

0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human EDAR(Accession Q9UNE0), corresponding to amino acid residues P265-T315.

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ectodysplasin A receptor , DL , ECTD10A , ECTD10B , ED1R , ED3 , ED5 , EDA-A1R , EDA1R , EDA3 , HRM1

Cellular Localization

Membrane

Background

This gene encodes a member of the tumor necrosis factor receptor family. The encoded transmembrane protein is a receptor for the soluble ligand ectodysplasin A, and can activate the nuclear factor-kappaB, JNK, and caspase-independent cell death pathways. It is required for the development of hair, teeth, and other ectodermal derivatives. Mutations in this gene result in autosomal dominant and recessive forms of hypohidrotic ectodermal dysplasia. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-EDAR antibody used in Western Blot (WB). GTX04637

GTX04637 WB Image

WB analysis of heat-shock treated COLO205 whole cell lysate using GTX04637 EDAR antibody.
Dilution : 1:1000

Anti-EDAR antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04637

GTX04637 ICC/IF Image

ICC/IF analysis PFA-fixed A549 cells using GTX04637 EDAR antibody.
Red : Primary antibody
Green : Beta tubulin
Blue : DAPI
Permeabilization : 0.1% Triton X-100

Anti-EDAR antibody used in IHC (Paraffin sections) (IHC-P). GTX04637

GTX04637 IHC-P Image

IHC-P analysis of mouse liver tissue using GTX04637 EDAR antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100

REFERENCE

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REVIEW

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Package List Price ($)
$ 399