GeneTex
United States (US)

EDC4 antibody

GTX115745_40269_IFA_18121410_456.jpg
GTX115745_40296_IHC_M_18121410_665.jpg
GTX115745_40296_WB_18121410_969.jpg
GTX115745_40296_WB_M_brain_18121410_813.jpg
GTX115745_40296_IHC_18121410_778.jpg

Cat No. GTX115745

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

152 kDa. ( Note )

Positive Control

NT2D1 , IMR32 , U87-MG , MCF-7 , mouse brain

Predict Reactivity

Rat(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human EDC4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Enhancer Of Mrna Decapping 4,Ge1,Ge-1,Hedl5,Hedls,Rcd-8,Rcd8,Edc4

Cellular Localization

Cytoplasm , P-body , Nucleus

Background

In the process of mRNA degradation, seems to play a role in mRNA decapping. Component of a complex containing DCP2 and DCP1A which functions in decapping of ARE-containing mRNAs. Promotes complex formation between DCP1A and DCP2. Enhances the catalytic activity of DCP2 (in vitro).

Database

DATA IMAGES
GTX115745_40269_IFA_18121410_456.jpg

GTX115745 ICC/IF Image

EDC4 antibody detects EDC4 protein at P-body by confocal immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EDC4 protein stained by EDC4 antibody (GTX115745) diluted at 1:500.
Blue: Hoechst 33343 staining.
[Images captured by Olympus FV10i Confocal Laser Scanning Microscope]

GTX115745_40296_IHC_M_18121410_665.jpg

GTX115745 IHC-P Image

EDC4 antibody detects EDC4 protein at cytosol and nucleus on mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
EDC4 antibody (GTX115745) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115745_40296_WB_18121410_969.jpg

GTX115745 WB Image

Sample (30 ug of whole cell lysate)
A: MCF-7
5% SDS PAGE
GTX115745 diluted at 1:3000

GTX115745_40296_WB_M_brain_18121410_813.jpg

GTX115745 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
5% SDS PAGE
GTX115745 diluted at 1:2000

GTX115745_40296_IHC_18121410_778.jpg

GTX115745 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using EDC4(GTX115745) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169