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EDC4 antibody

Anti-EDC4 antibody used in IHC (Paraffin sections) (IHC-P). GTX115745
Anti-EDC4 antibody used in Western Blot (WB). GTX115745
Anti-EDC4 antibody used in Western Blot (WB). GTX115745
Anti-EDC4 antibody used in IHC (Paraffin sections) (IHC-P). GTX115745
Anti-EDC4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115745

Cat. No. GTX115745

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($419),
25 μl ($169)
See all EDC4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

152 kDa. ( Note )

Positive Control

Mouse brain , MCF-7

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human EDC4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10624671

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

enhancer of mRNA decapping 4 , GE1 , Ge-1 , HEDL5 , HEDLS , RCD-8 , RCD8

Cellular Localization

Cytoplasm , P-body , Nucleus

Background

In the process of mRNA degradation, seems to play a role in mRNA decapping. Component of a complex containing DCP2 and DCP1A which functions in decapping of ARE-containing mRNAs. Promotes complex formation between DCP1A and DCP2. Enhances the catalytic activity of DCP2 (in vitro).

Database

Research Area

DATA IMAGES

Anti-EDC4 antibody used in IHC (Paraffin sections) (IHC-P). GTX115745

GTX115745 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using EDC4(GTX115745) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EDC4 antibody used in Western Blot (WB). GTX115745

GTX115745 WB Image

Sample (30 ug of whole cell lysate)
A: MCF-7
5% SDS PAGE
GTX115745 diluted at 1:3000

Anti-EDC4 antibody used in Western Blot (WB). GTX115745

GTX115745 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
5% SDS PAGE
GTX115745 diluted at 1:2000

Anti-EDC4 antibody used in IHC (Paraffin sections) (IHC-P). GTX115745

GTX115745 IHC-P Image

EDC4 antibody detects EDC4 protein at cytosol and nucleus on mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
EDC4 antibody (GTX115745) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EDC4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115745

GTX115745 ICC/IF Image

EDC4 antibody detects EDC4 protein at P-body by confocal immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EDC4 protein stained by EDC4 antibody (GTX115745) diluted at 1:500.
Blue: Hoechst 33343 staining.
[Images captured by Olympus FV10i Confocal Laser Scanning Microscope]

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 419
$ 169